Taub D, Dastych J, Inamura N, Upton J, Kelvin D, Metcalfe D, Oppenheim J
Clinical Services Program, National Cancer Institute-Frederick Cancer Research and Development Center, MD 21702.
J Immunol. 1995 Mar 1;154(5):2393-402.
We have determined that several chemokines induce mast cell migration in vitro. This directed migration is dependent on the presence of particular extracellular matrix proteins and the activation status of the cells. Mast cell haptotactic responses were observed in response to various chemokines on vitronectin-, laminin-, and fibronectin-coated filters. Unstimulated mast cells were chemoattracted only by monocyte chemotactic protein-1 and RANTES on vitronectin-coated and, to a lesser extent, laminin-coated filters, whereas IgE-activated mast cells migrated in response to monocyte chemotactic protein-1, regulated on activation normal T expressed and secreted, platelet factor-4, and macrophage inflammatory protein-1 alpha on all three matrix proteins. No significant migration was observed on collagen type IV-coated or uncoated filters. Mast cell migration in response to chemokines on extracellular matrices and its enhancement by IgE-dependent activation provide a mechanism by which cells may be drawn to sites of inflammation. Chemokine-induced mast cell recruitment may be particularly relevant in host defense responses to parasitic infections, allergic reactions, Jones-Mote reactions, and in wound healing.
我们已经确定，几种趋化因子在体外可诱导肥大细胞迁移。这种定向迁移取决于特定细胞外基质蛋白的存在以及细胞的激活状态。在玻连蛋白、层粘连蛋白和纤连蛋白包被的滤膜上，观察到肥大细胞对各种趋化因子产生趋触性反应。未受刺激的肥大细胞仅在玻连蛋白包被的滤膜上，以及在较小程度上在层粘连蛋白包被的滤膜上，被单核细胞趋化蛋白-1和RANTES趋化，而IgE激活的肥大细胞在所有三种基质蛋白上对单核细胞趋化蛋白-1、活化正常T细胞表达和分泌因子、血小板因子-4以及巨噬细胞炎性蛋白-1α产生迁移反应。在IV型胶原包被或未包被的滤膜上未观察到明显的迁移。肥大细胞在细胞外基质上对趋化因子的迁移反应及其由IgE依赖性激活所增强，提供了一种可将细胞吸引至炎症部位的机制。趋化因子诱导的肥大细胞募集在宿主对寄生虫感染、过敏反应、琼斯-莫特反应以及伤口愈合的防御反应中可能尤为重要。
