核糖体分型技术在鉴别泰国虾养殖场分离出的非O1群霍乱弧菌中的应用。
Application of ribotyping for differentiating Vibrio cholerae non-O1 isolated from shrimp farms in Thailand.
作者信息
Dalsgaard A, Echeverria P, Larsen J L, Siebeling R, Serichantalergs O, Huss H H
机构信息
Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
出版信息
Appl Environ Microbiol. 1995 Jan;61(1):245-51. doi: 10.1128/aem.61.1.245-251.1995.
Abstract
A collection of 143 Vibrio cholerae non-O1 strains isolated from shrimp farms in Thailand were characterized and grouped by ribotyping. Sixty-four ribotypes were distinguished following digestion of chromosomal DNA with the restriction enzyme BglI, and the reproducibility of the method was 100%. There was no correlation between specific ribotype distributions and the locations of the shrimp farms. Ribotype similarity was examined by cluster analysis, and two main groups with 10 and 54 ribotypes, respectively, were found. Correlation between ribotype and O-antigen expression was shown to exist among those isolates tested. Ribotyping appears to be a suitable method for differentiating environmental V. cholerae non-O1 strains, and comparison of ribotype patterns showed a high degree of genetic divergence within V. cholerae non-O1.
摘要
对从泰国虾养殖场分离出的143株霍乱弧菌非O1菌株进行了核糖体分型鉴定和分组。用限制性内切酶BglI消化染色体DNA后，区分出64种核糖体分型，该方法的重复性为100%。特定核糖体分型分布与虾养殖场的位置之间没有相关性。通过聚类分析检查核糖体分型相似性，发现了分别有10种和54种核糖体分型的两个主要组。在所测试的那些分离株中显示出核糖体分型与O抗原表达之间存在相关性。核糖体分型似乎是区分环境霍乱弧菌非O1菌株的合适方法，核糖体分型模式的比较显示霍乱弧菌非O1内存在高度的遗传差异。
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