Ogiso M, Okinaga T, Komoto M, Nishiyama I, Hoshi M
Cell and Information, Presto, Research Development Corporation of Japan (JRDC), Tokyo.
Exp Eye Res. 1994 Dec;59(6):653-63. doi: 10.1006/exer.1994.1151.
The carbohydrate epitope Gal alpha 1-3Gal-R (alpha-galactosyl epitope), which is detectable by its binding with Bandeiraea simplicifolia-IB4 lectin, was found in glycosphingolipids (GSLs), both neutral and acidic (gangliosides), from lens tissues of non-primate mammals, but not in those of human senile cataracts and Old World monkeys. Instead, human cataractous and Old World monkey non-cataractous lenses expressed Lewisx (Le(x)) epitopes (Gal beta 1-4(Fuc alpha 1-3)GlcNAc-R) in neutral GSLs. Sialylated Le(x) epitopes were found in rat and pig lenses as well as in human and Old World monkey lenses. Ganglio-series gangliosides, consisting mainly of GM3, GM1, GD1a and GD3, were detected in a species-specific fashion. On the other hand, alpha-galactosyl epitopes were expressed in lens tissues only in water-insoluble proteins of non-primate mammals, but Le(x) and sialylated Le(x) epitopes were not detectable in lens proteins. Among the several mammalian lenses examined, humans and Old World monkeys showed similar GSL compositions, in particular the presence of Le(x) and sialylated Le(x) epitopes and the absence of alpha-galactosyl epitopes, in lens tissue.
通过与单叶豆凝集素IB4结合可检测到的碳水化合物表位Galα1-3Gal-R（α-半乳糖基表位），在非灵长类哺乳动物晶状体组织的中性和酸性糖鞘脂（GSLs，神经节苷脂）中被发现，但在人类老年性白内障和旧世界猴的晶状体组织中未发现。相反，人类白内障晶状体和旧世界猴的非白内障晶状体在中性GSLs中表达Lewisx（Le(x)）表位（Galβ1-4(Fucα1-3)GlcNAc-R）。在大鼠和猪的晶状体以及人类和旧世界猴的晶状体中发现了唾液酸化的Le(x)表位。主要由GM3、GM1、GD1a和GD3组成的神经节系列神经节苷脂以物种特异性方式被检测到。另一方面，α-半乳糖基表位仅在非灵长类哺乳动物的水不溶性蛋白质中的晶状体组织中表达，但在晶状体蛋白质中未检测到Le(x)和唾液酸化的Le(x)表位。在所检测的几种哺乳动物晶状体中，人类和旧世界猴在晶状体组织中表现出相似的GSL组成，特别是存在Le(x)和唾液酸化的Le(x)表位以及不存在α-半乳糖基表位。
