Ogiso M, Takehana M, Kobayashi S, Hoshi M
Cell and Information, PRESTO, Japan Science and Technology Corporation (JST), Japan.
Exp Eye Res. 1998 Jun;66(6):765-73. doi: 10.1006/exer.1998.0489.
Monkey and human lenses contain essentially the same glycosphingolipids, and Lewisx and sialylated Lewisx epitopes are expressed on the terminal structure of neolactotetraosylceramide. However, monolayer cultures of lens epithelial cells from rhesus monkey expressed gangliosides GM3, GD3 and a small amount of GM1, but not sialylated Lewisx epitopes. Eight-week-old cultures on various extracellular matrices resulted in morphological changes in lens epithelial cells. Monolayer of cells cultured on vitronectin or polylysine assembled into aggregates after 4 weeks of culture. Cells cultured on vitronectin expressed sialyl-Lewisx gangliosides and did not exhibit GD3. On collagens, fibronectin and laminin elongated cells were observed in cells cultured for 8 weeks. Thus, the interaction between cells and extracellular matrices influenced morphology and glycosphingolipid composition in lens epithelial cells.
猴和人晶状体所含的糖鞘脂基本相同，Lewisx和唾液酸化Lewisx表位在新乳糖四糖神经酰胺的末端结构上表达。然而，恒河猴晶状体上皮细胞的单层培养物表达神经节苷脂GM3、GD3和少量GM1，但不表达唾液酸化Lewisx表位。在各种细胞外基质上培养8周导致晶状体上皮细胞形态发生变化。在玻连蛋白或聚赖氨酸上培养的单层细胞在培养4周后聚集成聚集体。在玻连蛋白上培养的细胞表达唾液酸化Lewisx神经节苷脂，且不表现出GD3。在胶原蛋白、纤连蛋白和层粘连蛋白上培养8周的细胞中观察到细胞伸长。因此，细胞与细胞外基质之间的相互作用影响了晶状体上皮细胞的形态和糖鞘脂组成。
