Ogiso M, Ohta M, Irie A, Hoshi M, Komoto M
Cell and Information, PRESTO, Research Development Corporation of Japan (JRDC).
Exp Eye Res. 1995 Feb;60(2):193-8. doi: 10.1016/s0014-4835(95)80010-7.
Neutral glycosphingolipids were purified from non-cataractous lenses of Sprague-Dawley rats by a combination of solvent extraction, Folch's partition, and column chromatography using DEAE-Sephadex and Iatrobeads. Six major GSLs from monohexosylceramide to pentahexosylceramide were identified by sugar composition analysis, methylation analysis and glycosidase digestion. Structural relationships among the six neutral glycosphingolipids revealed metabolic pathways leading to the synthesis of Gal alpha 1-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1 ceramide (IV3Gal alpha nLc4), instead of a Lewis(x) glycolipid (Gal beta 1- 4(Fuc alpha 1-3)GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1 ceramide, III3FucnLc4), from neolactotetraosylceramide (nLc4), together with isoglobotriaosylceramide (iGb3). The alpha-galactosyl epitope, Gal alpha 1-3Gal-R, is evolutionarily conserved in many types of cells of non-primate mammals, prosimians and New World monkeys, but not in those of Old World monkeys or humans. This evolution-related difference in carbohydrate epitopes suggests different cell-to-cell attachments, which may be mediated through cell surface glycosphingolipids, between rat and human lenses.
通过溶剂萃取、福尔克分配法以及使用二乙氨基乙基葡聚糖（DEAE - Sephadex）和离子交换树脂珠（Iatrobeads）的柱色谱法相结合，从斯普拉格 - 道利大鼠的非白内障晶状体中纯化出中性糖鞘脂。通过糖组成分析、甲基化分析和糖苷酶消化鉴定出了从单己糖神经酰胺到五己糖神经酰胺的六种主要糖鞘脂。六种中性糖鞘脂之间的结构关系揭示了从新乳糖四糖神经酰胺（nLc4）合成Galα1 - 3Galβ1 - 4GlcNAcβ1 - 3Galβ1 - 4Glcβ1 - 1神经酰胺（IV3GalαnLc4）的代谢途径，而不是从新乳糖四糖神经酰胺合成路易斯（x）糖脂（Galβ1 - 4（Fucα1 - 3）GlcNAcβ1 - 3Galβ1 - 4Glcβ1 - 1神经酰胺，III3FucnLc4），同时还合成了异球三糖神经酰胺（iGb3）。α - 半乳糖基表位Galα1 - 3Gal - R在非灵长类哺乳动物、原猴和新大陆猴的多种细胞类型中在进化上是保守的，但在旧大陆猴或人类的细胞中则不然。碳水化合物表位的这种与进化相关的差异表明大鼠和人类晶状体之间存在不同的细胞间附着方式，这可能是通过细胞表面糖鞘脂介导的。
