Jones T J, Coad N A, Muir K R, Parkes S E, Evans C D, Mann J R
Department of Histopathology, Royal Shrewsbury Hospital, Shropshire.
J Clin Pathol. 1995 Jan;48(1):22-5. doi: 10.1136/jcp.48.1.22.
To analyse the immunophenotype of a large number of non-endemic Burkitt's lymphomas to determine whether a B cell phenotype is consistently recognisable using formalin fixed, paraffin wax embedded archival material and a standard panel of commercially available antibodies.
Archival material was obtained from 30 cases of childhood Burkitt's lymphoma registered with the West Midlands Regional Children's Tumour Research Group. These were analysed by a standard avidin biotin complex immunoperoxidase method using antibodies to CD45, CD43, CD30, CD20, CD15, and immunoglobulin heavy and light chains.
There was a high incidence of the CD45RB and CD20 immunophenotypes, with a clearly recognisable B cell lineage even in archival material. IgM was identifiable in 13 of the 23 (56.5%) cases tested. Only three of 17 (18%) cases expressed CD30. Positive membrane staining with CD45RO was observed in two (6.7%) cases.
A B cell lineage can be identified in Burkitt's lymphoma in formalin fixed, paraffin wax embedded material, even in archival tissue. There was a low incidence of membrane staining with CD45RO which is a potential source of diagnostic confusion.
分析大量非地方性伯基特淋巴瘤的免疫表型，以确定使用福尔马林固定、石蜡包埋的存档材料和一组标准的市售抗体是否能一致识别B细胞表型。
存档材料取自西米德兰兹地区儿童肿瘤研究组登记的30例儿童伯基特淋巴瘤病例。使用针对CD45、CD43、CD30、CD20、CD15以及免疫球蛋白重链和轻链的抗体，通过标准的抗生物素蛋白-生物素复合物免疫过氧化物酶方法对这些病例进行分析。
CD45RB和CD20免疫表型的发生率很高，即使在存档材料中也能清楚地识别出B细胞谱系。在23例检测病例中的13例（56.5%）中可识别出IgM。17例病例中只有3例（18%）表达CD30。在2例（6.7%）病例中观察到CD45RO的阳性膜染色。
即使在存档组织中，在福尔马林固定、石蜡包埋的材料中也能在伯基特淋巴瘤中识别出B细胞谱系。CD45RO的膜染色发生率较低，这是诊断混淆的一个潜在来源。
