Payne C M, Grogan T M, Cromey D W, Bjore C G, Kerrigan D P
Lab Invest. 1987 Aug;57(2):200-18.
Six cases of Burkitt's lymphoma (BL) and 12 cases of Burkitt's-like lymphoma (BLL) were classified by using strict histologic and cytologic criteria. These cases were processed for electron microscopy and analyzed by using computerized image analysis techniques. Form factor (4 pi A/P2) was used to measure nuclear contour irregularity. The mean of the standard deviation (SD) of nuclear area and form factor was used to assess pleomorphism. Overall, there were 8 similarities and 10 statistically significant dissimilarities out of 18 parameters analyzed. The similarities (p greater than 0.05) between the BL and BLL groups included the means of form factor, nuclear area/cytoplasmic area, SD of nuclear area/cytoplasmic area, number of nuclear pockets/100 nuclei, percentage of cells with nuclear pockets, number of lipid droplets/micron 2 of cytoplasm, percentage of cells with lipid droplets and number of mitochondria/micron 2 of cytoplasm. The dissimilarities (p less than 0.05) included the means of nuclear perimeter, SD of nuclear perimeter, nuclear area, SD of nuclear area, cellular area, SD of cellular area, cytoplasmic area, SD of cytoplasmic area, SD of form factor, and nucleolar frequency. Multiparameter analysis clearly separated these 18 patients into two distinct groups and confirms that the subtleties used in the histologic classification of these lymphoma subtypes are meaningful. Sixteen cases of BL and BLL were snap-frozen in isopentane and analyzed by using 16 lymphoid surface markers. All of the immunoglobulin-positive BL were of the mu isotype, whereas the BLL cases were divided between mu (6 cases) and gamma expression (4 cases). All 4 of the BL evaluated manifested CALLA expression, whereas 3 of the 11 BLL evaluated coexpressed CALLA. One BL case was of a pre-pre-B phenotype and one BLL case was of pre-B phenotype. The BL and BLL were compared to 49 cases of SIg (+) large cell lymphomas. The high incidence of coexpression of lambda, CALLA, and Ki-67 in BL and BLL separates these lymphomas, as a group, from the large cell lymphomas. We have also determined from this study that the separation of patients into distinct BL and BLL subtypes is clinically relevant. The BL group were all children (median of 6.5 years) compared with the BLL group who were all adults (median of 63 years). The complete remission rate was higher in the BL (67%) than in the BLL group (25%).4off
采用严格的组织学和细胞学标准对6例伯基特淋巴瘤（BL）和12例伯基特样淋巴瘤（BLL）进行分类。对这些病例进行电子显微镜处理，并使用计算机图像分析技术进行分析。形状因子（4πA/P²）用于测量核轮廓不规则性。核面积和形状因子的标准差（SD）均值用于评估多形性。总体而言，在分析的18个参数中，有8个相似点和10个具有统计学意义的不同点。BL组和BLL组之间的相似点（p＞0.05）包括形状因子均值、核面积/细胞质面积、核面积/细胞质面积的SD、核袋数/100个核、有核袋细胞的百分比、脂滴数/细胞质平方微米、有脂滴细胞的百分比以及线粒体数/细胞质平方微米。不同点（p＜0.05）包括核周长均值、核周长的SD、核面积、核面积的SD、细胞面积、细胞面积的SD、细胞质面积、细胞质面积的SD、形状因子的SD以及核仁频率。多参数分析清楚地将这18例患者分为两个不同的组，并证实了这些淋巴瘤亚型组织学分类中所使用的细微差别是有意义的。16例BL和BLL病例在异戊烷中速冻，并使用16种淋巴样表面标志物进行分析。所有免疫球蛋白阳性的BL均为μ同种型，而BLL病例在μ表达（6例）和γ表达（4例）之间分布。评估的所有4例BL均表现出CALLA表达，而评估的11例BLL中有3例共表达CALLA。1例BL病例为前前B表型，1例BLL病例为前B表型。将BL和BLL与49例SIg（+）大细胞淋巴瘤进行比较。BL和BLL中λ、CALLA和Ki-67共表达的高发生率将这些淋巴瘤作为一个整体与大细胞淋巴瘤区分开来。我们还从这项研究中确定，将患者分为不同的BL和BLL亚型在临床上是相关的。BL组均为儿童（中位年龄6.5岁），而BLL组均为成年人（中位年龄63岁）。BL的完全缓解率（67%）高于BLL组（25%）。
