Bergmans A M, Groothedde J W, Schellekens J F, van Embden J D, Ossewaarde J M, Schouls L M
Molecular Microbiology Unit, National Institute of Public Health and Environmental Protection, Bilthoven, Netherlands.
J Infect Dis. 1995 Apr;171(4):916-23. doi: 10.1093/infdis/171.4.916.
To determine the role of Bartonella (formerly Rochalimaea) species and Afipia felis in cat scratch disease (CSD), two polymerase chain reaction (PCR) hybridization assays were developed to detect DNA from these organisms. These assays were applied on 89 pus aspirates from skin test-positive CSD patients (group 1) and on 137 pus and lymph node specimens from CSD suspects (group 2). Bartonella DNA was detected in 96% of the samples from group 1 patients and in 60% of group 2 samples; however, A. felis DNA could not be detected in any clinical samples. These results suggest that CSD is caused by bartonellae and that A. felis does not play a significant role in this zoonosis. A strong correlation between Bartonella PCR positivity and Bartonella henselae antibody titer was found. Comparison of CSD skin test results with those obtained by Bartonella PCR suggests a low sensitivity of the skin test.
为确定巴尔通体（原罗卡利马氏体）属菌种和阿菲彼亚猫菌在猫抓病（CSD）中的作用，开发了两种聚合酶链反应（PCR）杂交检测法以检测这些微生物的DNA。这些检测法应用于89例皮肤试验呈阳性的CSD患者的脓液抽吸物（第1组）以及137例CSD疑似患者的脓液和淋巴结标本（第2组）。在第1组患者的96%样本以及第2组60%的样本中检测到了巴尔通体DNA；然而，在任何临床样本中均未检测到阿菲彼亚猫菌DNA。这些结果表明，CSD是由巴尔通体引起的，且阿菲彼亚猫菌在这种人畜共患病中不起重要作用。发现巴尔通体PCR阳性与汉赛巴尔通体抗体滴度之间存在强相关性。将CSD皮肤试验结果与通过巴尔通体PCR获得的结果进行比较，表明皮肤试验的敏感性较低。
