Giladi M, Avidor B, Kletter Y, Abulafia S, Slater L N, Welch D F, Brenner D J, Steigerwalt A G, Whitney A M, Ephros M
The Bernard Pridan Laboratory for Molecular Biology of Infectious Diseases, Ichilov Hospital, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel.
J Clin Microbiol. 1998 Sep;36(9):2499-502. doi: 10.1128/JCM.36.9.2499-2502.1998.
Since its isolation in 1988, Afipia felis has been associated with cat scratch disease (CSD) in only one report and its role in CSD has been questioned. We have cultured A. felis from a lymph node of a patient with CSD. 16S rRNA gene sequencing, DNA relatedness studies, fatty acid analysis, and PCR of the A. felis ferredoxin gene showed that the isolate is identical to the previously reported A. felis isolate. To determine the role of A. felis in CSD, PCR of the 16S rRNA gene followed by hybridizations with specific probes were performed with lymph node specimens from CSD patients. All 32 specimens tested positive for Bartonella henselae and negative for A. felis. We conclude that A. felis is a rare cause of CSD. Diagnostic tests not conducive to the identification of A. felis might cause the diagnosis of CSD due to A. felis to be missed.
自1988年分离出以来,费氏阿菲波菌仅在一份报告中与猫抓病(CSD)相关联,其在CSD中的作用一直受到质疑。我们从一名CSD患者的淋巴结中培养出了费氏阿菲波菌。16S rRNA基因测序、DNA相关性研究、脂肪酸分析以及费氏阿菲波菌铁氧化还原蛋白基因的PCR检测表明,该分离株与先前报道的费氏阿菲波菌分离株相同。为了确定费氏阿菲波菌在CSD中的作用,我们对CSD患者的淋巴结标本进行了16S rRNA基因的PCR检测,随后用特异性探针进行杂交。所有32份检测标本均对汉赛巴尔通体呈阳性,对费氏阿菲波菌呈阴性。我们得出结论,费氏阿菲波菌是CSD的罕见病因。不利于费氏阿菲波菌鉴定的诊断测试可能会导致漏诊由费氏阿菲波菌引起的CSD。