Ravot G, Magot M, Fardeau M L, Patel B K, Prensier G, Egan A, Garcia J L, Ollivier B
Laboratoire de Microbiologie ORSTOM, Université de Provence, Marseille, France.
Int J Syst Bacteriol. 1995 Apr;45(2):308-14. doi: 10.1099/00207713-45-2-308.
A thermophilic, glucose-fermenting, strictly anaerobic, rod-shaped bacterium, strain SEBR 6459T (T = type strain), was isolated from an African oil-producing well. This organism was identified as a member of the genus Thermotoga on the basis of the presence of the typical outer sheath-like structure (toga) and 16S rRNA signature sequences and its ability to grow on carbohydrates (glucose, arabinose, fructose, lactose, maltose, and xylose). Major differences in its 16S rRNA gene sequence, its lower optimum temperature for growth (66 degrees C), its sodium chloride range for growth (0 to 2.8%), its lack of lactate as an end product from glucose fermentation, and its peritrichous flagella indicate that strain SEBR 6459T is not similar to the three previously described Thermotoga species. Furthermore, this organism does not belong to any of the other genera related to the order Thermotogales that have been described. On the basis of these findings, we propose that this strain should be described as a new species, Thermotoga elfii. The type strain of T. elfii is SEBR 6459 (= DSM 9442).
从一口非洲产油井中分离出了一株嗜热、能发酵葡萄糖、严格厌氧的杆状细菌，菌株SEBR 6459T（T = 模式菌株）。基于典型的鞘状外层结构（toga）和16S rRNA特征序列的存在以及其在碳水化合物（葡萄糖、阿拉伯糖、果糖、乳糖、麦芽糖和木糖）上生长的能力，该生物体被鉴定为嗜热栖热菌属的成员。其16S rRNA基因序列的主要差异、较低的最适生长温度（66℃）、生长的氯化钠范围（0至2.8%）、缺乏作为葡萄糖发酵终产物的乳酸以及其周生鞭毛表明菌株SEBR 6459T与先前描述的三种嗜热栖热菌属物种不同。此外，该生物体不属于已描述的与嗜热栖热菌目相关的任何其他属。基于这些发现，我们提议将该菌株描述为一个新物种，即埃尔菲嗜热栖热菌。埃尔菲嗜热栖热菌的模式菌株是SEBR 6459（= DSM 9442）。
