Kim S B, Yoon J H, Kim H, Lee S T, Park Y H, Goodfellow M
Department of Microbiology, Medical School, Newcastle upon Tyne, United Kingdom.
Int J Syst Bacteriol. 1995 Apr;45(2):351-6. doi: 10.1099/00207713-45-2-351.
Nearly complete sequences of 16S rRNA genes of representative strains of the genus Saccharomonospora were determined following the isolation and cloning of the amplified genes. The sequences were aligned with those of representatives of the family Pseudonocardiaceae, and a phylogenetic tree was inferred by the neighbor-joining method. The genus Saccharomonospora formed a distinct clade within the evolutionary radiation encompassed by the family Pseudonocardiaceae. The average nucleotide similarity value found between the type strains of the four validly described Saccharomonospora species was 97.5% +/- 1.0%. The most distant relationship was found between Saccharomonospora azurea and Saccharomonospora viridis K73 (96.3% similarity). In contrast, Saccharomonospora azurea K161 and "Saccharomonospora caesia" K163 had identical 16S rRNA gene sequences. The nucleotide sequence data suggest that the genus Saccharomonospora contains several new centers of variation.
在对扩增基因进行分离和克隆后，测定了糖单孢菌属代表性菌株的16S rRNA基因的几乎完整序列。将这些序列与假诺卡氏菌科代表菌株的序列进行比对，并通过邻接法推断出系统发育树。糖单孢菌属在假诺卡氏菌科所涵盖的进化辐射范围内形成了一个独特的分支。在四个有效描述的糖单孢菌物种的模式菌株之间发现的平均核苷酸相似性值为97.5%±1.0%。在天蓝糖单孢菌和绿色糖单孢菌K73之间发现了最远的亲缘关系（相似性为96.3%）。相比之下，天蓝糖单孢菌K161和“蓝色糖单孢菌”K163具有相同的16S rRNA基因序列。核苷酸序列数据表明，糖单孢菌属包含几个新的变异中心。
