Cho M, Yoon J H, Kim S B, Park Y H
Korean Collection for Type Cultures (KCTC), Korea Research Intitute of Bioscience and Biotechnology (KRIBB), Yusong, Taejon, Korea.
Int J Syst Bacteriol. 1998 Oct;48 Pt 4:1223-30. doi: 10.1099/00207713-48-4-1223.
Sequences of the RNase P RNA gene were investigated for the phylogenetic analysis of the genus Saccharomonospora. Aligned nucleotide sequences, determined from the PCR-amplified RNase P RNA gene of representative strains of the genus Saccharomonospora, displayed 94.2 +/- 1.3% interspecific variances. The intraspecific similarity value was 99.7-100% in all species tested. Saccharomonospora azurea K161T and 'Saccharomonospora caesia' K76T displayed identical RNase P RNA gene sequences in the region that was determined and Saccharomonospora sp. K180 showed sequences distinct from validly described species with a similarity value of 94.6 +/- 1.0%. The phylogenetic trees constructed by aligning the sequences either within the genus Saccharomonospora or with other Gram-positive bacteria were similar to the ones derived using sequences of the 16S rDNA gene. Advantageous features of this gene for application as a molecular phyletic marker are discussed.
对核糖核酸酶P RNA基因序列进行了研究，以用于糖单孢菌属的系统发育分析。从糖单孢菌属代表性菌株的PCR扩增核糖核酸酶P RNA基因中确定的比对核苷酸序列显示，种间差异为94.2±1.3%。在所有测试物种中，种内相似性值为99.7 - 100%。天蓝糖单孢菌K161T和“浅蓝糖单孢菌”K76T在所测定区域显示出相同的核糖核酸酶P RNA基因序列，糖单孢菌属菌株K180显示出与有效描述物种不同的序列，相似性值为94.6±1.0%。通过比对糖单孢菌属内序列或与其他革兰氏阳性菌序列构建的系统发育树，与使用16S rDNA基因序列得到的系统发育树相似。讨论了该基因作为分子系统发育标记应用的有利特征。
