肠出血性大肠杆菌O157:H7需要紧密黏附素才能在无菌猪肠道中定殖并黏附于HEp-2细胞。
Enterohemorrhagic Escherichia coli O157:H7 requires intimin to colonize the gnotobiotic pig intestine and to adhere to HEp-2 cells.
作者信息
McKee M L, Melton-Celsa A R, Moxley R A, Francis D H, O'Brien A D
机构信息
Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, F. Edward Hébert School of Medicine, Bethesda, Maryland 20814-4799, USA.
出版信息
Infect Immun. 1995 Sep;63(9):3739-44. doi: 10.1128/iai.63.9.3739-3744.1995.
Abstract
In a previous study, enterohemorrhagic Escherichia coli (EHEC) O157:H7 with a deletion and insertion in the eaeA gene encoding intimin was used to establish that intimin is required for the organism to attach to and efface microvilli in the piglet intestine (M. S. Donnenberg, S. Tzipori, M. L. McKee, A. D. O'Brien, J. Alroy, and J. B. Kaper, J. Clin. Invest. 92:1418-1424, 1993). However, in the same investigation, a role for intimin in EHEC adherence to HEp-2 cells could not be definitively demonstrated. To analyze the basis for this discrepancy, we constructed an in-frame deletion of eaeA and compared the adherence capacity of this mutant with that of the wild-type strain in vitro and in vivo. We observed a direct correlation between the requisite for intimin in EHEC O157:H7 colonization of the gnotobiotic piglet intestine and adherence of the bacterium to HEp-2 cells. The in vitro-in vivo correlation lends credence to the use of the HEp-2 cell adherence model for further study of the intimin protein.
摘要
在先前的一项研究中，使用在编码紧密黏附素的eaeA基因中存在缺失和插入的肠出血性大肠杆菌（EHEC）O157:H7来证实紧密黏附素是该生物体附着并抹平仔猪肠道微绒毛所必需的（M. S. 唐嫩伯格、S. 齐波里、M. L. 麦基、A. D. 奥布赖恩、J. 阿尔罗伊和J. B. 卡珀，《临床研究杂志》92:1418 - 1424, 1993）。然而，在同一研究中，紧密黏附素在EHEC黏附HEp - 2细胞中的作用未能得到明确证实。为了分析这种差异的原因，我们构建了eaeA的框内缺失，并在体外和体内比较了该突变体与野生型菌株的黏附能力。我们观察到紧密黏附素对于EHEC O157:H7在无菌仔猪肠道定殖的必要性与该细菌对HEp - 2细胞的黏附之间存在直接关联。体外 - 体内的相关性为使用HEp - 2细胞黏附模型进一步研究紧密黏附素蛋白提供了可信度。
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