Olinski R, Zastawny T H, Foksinski M, Barecki A, Dizdaroglu M
Department of Clinical Biochemistry, Medical Academy, Bydgoszcz, Poland.
Free Radic Biol Med. 1995 Apr;18(4):807-13. doi: 10.1016/0891-5849(94)00171-f.
The authors have studied DNA base damage and activities of antioxidant enzymes in human benign prostatic hyperplasia (BPH) tissues and surrounding disease-free tissues removed from prostate glands of 15 patients. In these tissues, endogenous levels of various typical hydroxyl radical-induced products of DNA bases and activities of catalase and superoxide dismutase were measured. The majority of patients had higher levels of DNA base lesions and lower activities of enzymes in BPH tissues than in normal prostate tissues. When activities of both enzymes were lower in BPH tissues than in normal tissues, the increases in the amounts of DNA base lesions over control levels were most prominent. In the case of similar enzyme activities in both BPH and normal tissues, no changes in levels of DNA base lesions were observed. These results suggest a possible association between antioxidant enzyme activities and levels of DNA base lesions in BPH tissues. Some of the identified DNA lesions are known to be premutagenic and may play a role in carcinogenesis. Although a possible link between BPH and prostate cancer is controversial, BPH patients with both decreased antioxidant enzyme activities and increased levels of DNA lesions may be at risk of developing prostate cancer.
作者研究了15例患者前列腺切除组织中，人类良性前列腺增生（BPH）组织及周围无病变组织中的DNA碱基损伤和抗氧化酶活性。测定了这些组织中各种典型的由羟基自由基诱导产生的DNA碱基产物的内源性水平，以及过氧化氢酶和超氧化物歧化酶的活性。大多数患者的BPH组织中DNA碱基损伤水平高于正常前列腺组织，而酶活性则低于正常前列腺组织。当BPH组织中两种酶的活性均低于正常组织时，DNA碱基损伤量相对于对照水平的增加最为显著。在BPH组织和正常组织中酶活性相似的情况下，未观察到DNA碱基损伤水平的变化。这些结果表明，抗氧化酶活性与BPH组织中DNA碱基损伤水平之间可能存在关联。一些已确定的DNA损伤已知是致突变前体，可能在致癌过程中起作用。尽管BPH与前列腺癌之间的可能联系存在争议，但抗氧化酶活性降低且DNA损伤水平升高的BPH患者可能有患前列腺癌的风险。
