Jaruga P, Zastawny T H, Skokowski J, Dizdaroglu M, Olinski R
Department of Clinical Biochemistry, Medical School, Bydgoszcz, Poland.
FEBS Lett. 1994 Mar 14;341(1):59-64. doi: 10.1016/0014-5793(94)80240-8.
We have investigated levels of antioxidant enzymes and free radical-induced DNA base modifications in human cancerous lung tissues and in their cancer-free surrounding tissues. Various DNA base lesions in chromatin of lung tissues were measured by gas chromatography-mass spectrometry. Activities of superoxide dismutase, catalase and glutathione peroxidase were also measured in lung tissues. Higher levels of DNA lesions were observed in cancerous tissues than in cancer-free surrounding tissues. Antioxidant enzyme levels were lower in cancerous tissues. The results indicate an association between decreased activities of antioxidant enzymes and increased levels of DNA lesions in cancerous tissues. Higher levels of DNA lesions suggest that free radical reactions may be increased in malignant tumor cells.
我们研究了人类肺癌组织及其癌旁无癌组织中抗氧化酶的水平以及自由基诱导的DNA碱基修饰情况。通过气相色谱-质谱法测定了肺组织染色质中的各种DNA碱基损伤。还测定了肺组织中超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性。癌组织中的DNA损伤水平高于癌旁无癌组织。癌组织中的抗氧化酶水平较低。结果表明，癌组织中抗氧化酶活性降低与DNA损伤水平升高之间存在关联。较高的DNA损伤水平表明恶性肿瘤细胞中的自由基反应可能增加。
