Mara Suburo A, Chaud M, Franchi A, Polak J M, Gimeno M A
Instituto de Neurobiología, Buenos Aires, Argentina.
Biol Reprod. 1995 Mar;52(3):631-7. doi: 10.1095/biolreprod52.3.631.
Since pharmacological evidence indicates that nitric oxide (NO) operates in the control of uterine motility, we have studied the distribution of NADPH diaphorase and NO synthases in the rat uterus using histochemical and immunohistochemical methods. Numerous nerve fibers displayed NADPH diaphorase activity and immunoreactivity to antisera raised against neuronal NO synthase. Nerve fibers appeared in all stages of the estrous cycle and also after ovariectomy. NADPH diaphorase activity was also present in endothelia and cells dispersed in the different uterine layers. Most NADPH diaphorase-positive (ND) cells had eosinophilic granules with occasional cells expressing the ED1 macrophage-monocyte marker. Immunoreactivity for an inducible NO synthase was found in a small number of macrophage-like cells without NADPH diaphorase activity. Thus, ND cells may express another NO synthase isoform not detected by the available antisera. In normal cycling rats, ND cells were most abundant during proestrus, and their number further increased after estrogen treatment. ND cells were not observed after ovariectomy but were present after estrogen replacement therapy. ND cells could be involved in the estrogenic control of in vivo and in vitro uterine.
由于药理学证据表明一氧化氮（NO）参与子宫运动的调控，我们采用组织化学和免疫组织化学方法研究了大鼠子宫中NADPH黄递酶和一氧化氮合酶的分布。许多神经纤维显示出NADPH黄递酶活性，并对针对神经元型一氧化氮合酶产生的抗血清具有免疫反应性。神经纤维出现在发情周期的各个阶段以及卵巢切除术后。NADPH黄递酶活性也存在于内皮细胞和分散在子宫不同层的细胞中。大多数NADPH黄递酶阳性（ND）细胞含有嗜酸性颗粒，偶尔有细胞表达ED1巨噬细胞-单核细胞标志物。在少数没有NADPH黄递酶活性的巨噬样细胞中发现了诱导型一氧化氮合酶的免疫反应性。因此，ND细胞可能表达了现有抗血清未检测到的另一种一氧化氮合酶同工型。在正常发情周期的大鼠中，ND细胞在发情前期最为丰富，雌激素处理后其数量进一步增加。卵巢切除术后未观察到ND细胞，但雌激素替代治疗后出现。ND细胞可能参与体内和体外子宫的雌激素调控。
