Tervo K, Latvala T, Suomalainen V P, Tervo T, Immonen I
Department of Anatomy, University of Helsinki, Finland.
Graefes Arch Clin Exp Ophthalmol. 1995 Mar;233(3):168-72. doi: 10.1007/BF00166610.
Posterior perforating eye injury carries a high risk of visual loss due to the formation of intravireal and epiretinal scar tissue. Intraocular scar formation in patients with retinal detachment has been shown to be associated with elevated intravitreal FN levels. The extracellular matrix glycoproteins fibronectin (FN) and tenascin (TN) have been located in epiretinal scar membranes. As both FN and TN are also involved in healing of cutaneous and corneal wounds, we undertook to study their expression in rabbit perforating scleral wounds with vitreous incarceration.
A perforating scleral wound was produced and sutured without removal of vitreous from the wound in 18 pigmented rabbits. The rabbits were killed at various times (1 h to 21 days) after the operation, and the indirect immunohistochemical method was used for demonstration of FN and TN. Monoclonal mouse hybridoma antibodies 52 DH1 and 100 EB2, recognizing the cellular form of FN (cFN) and TN, respectively, were used.
During the first post-operative week immunoreaction for glycoproteins, both the locally produced cFN and TN, were observed at the scar tissue containing the prolabed vitreous and the adjacent sclera. Subsequently, the reaction gradually shifted to the vitreal side of the wound, and 3 weeks after the operation it was almost completely restricted to a separated mass of vitreous beneath the scar.
The expression of cFN and TN in the scleral scar and vitreous is indicative of their local synthesis. The shift of the expression of those proteins to the vitreal side of the wound with time suggests that the scarring process in the vitreous is delayed compared to the sclera.
由于玻璃体内和视网膜前瘢痕组织的形成，后部眼球穿孔伤导致视力丧失的风险很高。视网膜脱离患者的眼内瘢痕形成已被证明与玻璃体内纤连蛋白（FN）水平升高有关。细胞外基质糖蛋白纤连蛋白（FN）和腱生蛋白（TN）已定位在视网膜前瘢痕膜中。由于FN和TN也参与皮肤和角膜伤口的愈合，我们着手研究它们在伴有玻璃体嵌顿的兔巩膜穿孔伤中的表达。
在18只有色兔中制造巩膜穿孔伤并缝合，不清除伤口处的玻璃体。术后在不同时间点（1小时至21天）处死兔子，采用间接免疫组织化学方法检测FN和TN。分别使用识别细胞形式的FN（cFN）和TN的单克隆小鼠杂交瘤抗体52 DH1和100 EB2。
术后第一周，在含有前房玻璃体的瘢痕组织和相邻巩膜处观察到糖蛋白的免疫反应，包括局部产生的cFN和TN。随后，反应逐渐转移到伤口的玻璃体一侧，术后3周，反应几乎完全局限于瘢痕下方分离的玻璃体团块。
cFN和TN在巩膜瘢痕和玻璃体中的表达表明它们在局部合成。随着时间的推移，这些蛋白质的表达向伤口的玻璃体侧转移，这表明玻璃体中的瘢痕形成过程比巩膜延迟。
