Hubner R J, Cole E M, Bruce J L, McDowell C I, Webster J A
Central Research and Development, E. I. du Pont de Nemours and Company, Wilmington, DE 19880, USA.
Proc Natl Acad Sci U S A. 1995 May 23;92(11):5234-8. doi: 10.1073/pnas.92.11.5234.
By using taxonomic characters derived from EcoRI restriction endonuclease digestion of genomic DNA and hybridization with a labeled rRNA operon from Escherichia coli, a polymorphic structure of Listeria monocytogenes, characterized by fragments with different frequencies of occurrence, was observed. This structure was expanded by creating predicted patterns through a recursive process of observation, expectation, prediction, and assessment of completeness. This process was applied, in turn, to normalized strain patterns, fragment bands, and positions of EcoRI recognition sites relative to rRNA regions. Analysis of 1346 strains provided observed patterns, fragment sizes, and their frequencies of occurrence in the patterns. Fragment size statistics led to the creation of unobserved combinations of bands, predicted pattern types. The observed fragment bands revealed positions of EcoRI sites relative to rRNA sequences. Each EcoRI site had a frequency of occurrence, and unobserved fragment sizes were postulated on the basis of knowing the restriction site locations. The result of the recursion process applied to the components of the strain data was an extended classification with observed and predicted members.
通过使用从基因组DNA的EcoRI限制性内切酶消化中获得的分类学特征，并与来自大肠杆菌的标记rRNA操纵子进行杂交，观察到了单核细胞增生李斯特菌的多态结构，其特征是具有不同出现频率的片段。通过观察、预期、预测和完整性评估的递归过程创建预测模式，扩展了这种结构。这个过程依次应用于标准化菌株模式、片段条带以及EcoRI识别位点相对于rRNA区域的位置。对1346株菌株的分析提供了观察到的模式、片段大小及其在模式中的出现频率。片段大小统计导致创建了未观察到的条带组合，即预测模式类型。观察到的片段条带揭示了EcoRI位点相对于rRNA序列的位置。每个EcoRI位点都有一个出现频率，并且基于已知的限制性位点位置推测未观察到的片段大小。应用于菌株数据组件的递归过程的结果是一个具有观察到的和预测的成员的扩展分类。
