Marounek M, Vovk S J, Skrivanová V
Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Prague.
Br J Nutr. 1995 Mar;73(3):463-9. doi: 10.1079/bjn19950048.
Activities of twelve hydrolytic enzymes in the digestive tract of young rabbits before weaning (4 weeks old) and adult rabbits (3 months old) were measured. The principal digestive enzymes in both groups of rabbits appeared to be amylase (EC 3.2.1.1), maltase (EC 3.2.1.20), pectinase (EC 3.2.1.15) and proteinases. The stomach of young rabbits contained most of the lipolytic activity and 45.7% of the total proteolytic activity of the digestive tract. The highest specific activities (per g digesta) of amylase, maltase and proteinase in young rabbits were found in the small intestine. Total activities (per segment) of amylase and maltase in the small intestine and the caecum were similar. Activities of cellulase (EC 3.2.1.4), inulinase (EC 3.2.1.7) and beta-glucosidase (EC 3.2.1.21) were low and activity of pectinase was fairly high in all segments of the digestive tract. The highest activity of urease (EC 3.5.1.5) was found in the caecum. Enzymic profiles of the colonic chymus resembled those of the caecum. Total hydrolytic activity was lower in the colon than in the caecum. Specific activities of amylase and invertase (EC 3.2.1.26) were lower and those of inulinase and lactase (EC 3.2.1.23) higher in 4-week-old rabbits than in 3-month-old rabbits. Gastric proteinase represented almost half of the total proteolytic activity of the digestive tract, whereas lipolytic activity of gastric contents was not found in measurable quantities in adult rabbits. The caecal contents of adult rabbits contained most of the total activity of lipase (EC 3.1.1.3), cellulase, xylanase (EC 3.2.1.32), pectinase, lactase, invertase, beta-glucosidase and urease present in the digestive tract.(ABSTRACT TRUNCATED AT 250 WORDS)
对断奶前幼兔（4周龄）和成兔（3月龄）消化道中12种水解酶的活性进行了测定。两组兔子的主要消化酶似乎都是淀粉酶（EC 3.2.1.1）、麦芽糖酶（EC 3.2.1.20）、果胶酶（EC 3.2.1.15）和蛋白酶。幼兔胃中含有消化道大部分的脂肪分解活性以及45.7%的总蛋白水解活性。幼兔小肠中淀粉酶、麦芽糖酶和蛋白酶的比活性（每克消化物）最高。小肠和盲肠中淀粉酶和麦芽糖酶的总活性（每段）相似。消化道各段中纤维素酶（EC 3.2.1.4）、菊粉酶（EC 3.2.1.7）和β-葡萄糖苷酶（EC 3.2.1.21）的活性较低，而果胶酶的活性相当高。脲酶（EC 3.5.1.5）的最高活性出现在盲肠中。结肠食糜的酶谱与盲肠相似。结肠中的总水解活性低于盲肠。4周龄兔子的淀粉酶和转化酶（EC 3.2.1.26）比活性低于3月龄兔子，而菊粉酶和乳糖酶（EC 3.2.1.23）比活性则更高。胃蛋白酶占消化道总蛋白水解活性的近一半，而成兔胃内容物中未检测到可测量的脂肪分解活性。成年兔盲肠内容物含有消化道中脂肪酶（EC 3.1.1.3）、纤维素酶、木聚糖酶（EC 3.2.1.32）、果胶酶、乳糖酶、转化酶、β-葡萄糖苷酶和脲酶的大部分总活性。（摘要截断于250字）
