Auld V J, Fetter R D, Broadie K, Goodman C S
Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA.
Cell. 1995 Jun 2;81(5):757-67. doi: 10.1016/0092-8674(95)90537-5.
Peripheral glia help ensure that motor and sensory axons are bathed in the appropriate ionic and biochemical environment. In Drosophila, peripheral glia help shield these axons against the high K+ concentration of the hemolymph, which would largely abolish their excitability. Here, we describe the molecular genetic analysis of gliotactin, a novel transmembrane protein that is transiently expressed on peripheral glia and that is required for the formation of the peripheral blood-nerve barrier. In gliotactin mutant embryos, the peripheral glia develop normally in many respects, except that ultrastructurally and physiologically they do not form a complete blood-nerve barrier. As a result, peripheral motor axons are exposed to the high K+ hemolymph, action potentials fail to propagate, and the embryos are nearly paralyzed.
外周神经胶质细胞有助于确保运动和感觉轴突沐浴在合适的离子和生化环境中。在果蝇中，外周神经胶质细胞有助于保护这些轴突免受血淋巴中高钾浓度的影响，否则轴突的兴奋性将基本丧失。在此，我们描述了神经胶质触蛋白的分子遗传学分析，神经胶质触蛋白是一种新的跨膜蛋白，在外周神经胶质细胞上短暂表达，是外周血-神经屏障形成所必需的。在神经胶质触蛋白突变体胚胎中，外周神经胶质细胞在许多方面发育正常，但在超微结构和生理功能上，它们无法形成完整的血-神经屏障。结果，外周运动轴突暴露于高钾血淋巴中，动作电位无法传导，胚胎几乎瘫痪。
