Li H P, Liu Z M, Nirenberg M
Laboratory of Biochemical Genetics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-4036, USA.
Proc Natl Acad Sci U S A. 1997 Feb 18;94(4):1086-91. doi: 10.1073/pnas.94.4.1086.
Kinesin-73 cDNA was shown to encode a kinesin heavy chain protein that contains an N-terminal motor domain and a long central region that lacks extensive coiled-coils. The amino acid sequence of the motor domain of kinesin-73 protein is most closely related to the motor domains of Caenorhabditis elegans unc-104 and mouse KIF1A. The central region of kinesin-73 protein also is related to unc-104 and KIF1A, but the homology is lower than that of the motor domain. The C-terminal region of kinesin-73 protein contains a cytoskeleton associated protein Gly-rich domain, which is a putative microtubule binding site that is present in some cytoskeleton or dynein-associated proteins. Kinesin-73 mRNA was shown by in situ hybridization to be maternally expressed and widely distributed in the syncytial blastoderm embryo. However, later in Drosophila embryo development, expression of the kinesin-73 gene becomes restricted mostly to the central and peripheral nervous systems.
驱动蛋白-73的cDNA被证明编码一种驱动蛋白重链蛋白，该蛋白包含一个N端运动结构域和一个缺乏广泛卷曲螺旋的长中央区域。驱动蛋白-73蛋白运动结构域的氨基酸序列与秀丽隐杆线虫unc-104和小鼠KIF1A的运动结构域关系最为密切。驱动蛋白-73蛋白的中央区域也与unc-104和KIF1A相关，但同源性低于运动结构域。驱动蛋白-73蛋白的C端区域包含一个富含甘氨酸的细胞骨架相关蛋白结构域，这是一个假定的微管结合位点，存在于一些细胞骨架或动力蛋白相关蛋白中。原位杂交显示，驱动蛋白-73 mRNA由母体表达，并广泛分布于合胞体胚盘胚胎中。然而，在果蝇胚胎发育后期，驱动蛋白-73基因的表达大多局限于中枢和外周神经系统。
