Kalhoff H, Werkmeister F, Kiwull-Schöne H, Diekmann L, Manz F, Kiwull P
Department of Physiology, Ruhr-University, Bochum, Germany.
Adv Exp Med Biol. 1994;361:353-61. doi: 10.1007/978-1-4615-1875-4_55.
The Haldane effect (HE) was investigated in human adults and prematures under normal metabolic acid-base conditions but at different levels of PCO2. Venous blood samples were equilibrated with low and high PCO2 in either O2 or N2. The change in plasma pH of oxygenated blood by deoxygenation did not differ between both groups. Thus, ontogenetic differences of human hemoglobin structure do not influence the net proton Haldane effect measured in terms of whole blood pH-changes. Since the present data quantitatively agree with those we reported earlier for rabbits, cats and dogs (Kiwull-Schöne et al., 1992), phylogenetic differences in hemoglobin structure of these mammalian species do not either seem to play a role in this respect. The influence of the Haldane effect on plasma pH has to be considered in blood-gas and acid-base analysis of samples with incomplete oxygenation. This is important for the indirect determination of PCO2 through pH by the equilibration method (Astrup and Schrøder, 1956), serving as reference method for determination of metabolic acid-base status and CO2 buffering capacity. Likewise, HE-correction is important for indirect estimation of metabolic acid-base status (BE and HCO-3st) from clinical routine PCO2- and pH-measurement. In spite of the vaste amount of literature on the Haldane effect in human blood, quantitative data for practical purpose are less available and still equivocal. By the present study, a strong inverse linear correlation between the HE-induced delta pH and 1g[HCO3-] could be shown over a wide range of acid-base changes.(ABSTRACT TRUNCATED AT 250 WORDS)
在正常代谢酸碱条件下，但处于不同PCO₂水平时，对成年人类和早产儿的哈代效应（HE）进行了研究。静脉血样本在O₂或N₂中用低PCO₂和高PCO₂进行平衡。两组中脱氧导致的氧合血血浆pH变化并无差异。因此，人类血红蛋白结构的个体发育差异不会影响根据全血pH变化测得的净质子哈代效应。由于目前的数据在定量上与我们之前报道的兔子、猫和狗的数据一致（Kiwull-Schöne等人，1992年），这些哺乳动物物种血红蛋白结构的系统发育差异在这方面似乎也不起作用。在对氧合不完全的样本进行血气和酸碱分析时，必须考虑哈代效应对血浆pH的影响。这对于通过平衡法（Astrup和Schrøder，1956年）通过pH间接测定PCO₂很重要，该方法用作测定代谢酸碱状态和CO₂缓冲能力的参考方法。同样，HE校正对于从临床常规PCO₂和pH测量中间接估计代谢酸碱状态（碱剩余和标准碳酸氢盐）也很重要。尽管关于人类血液中哈代效应的文献数量众多，但实际应用的定量数据较少且仍不明确。通过本研究，在广泛的酸碱变化范围内，可以显示出HE诱导的δpH与1g[HCO₃⁻]之间存在强烈的负线性相关性。（摘要截于250字）
