Phuchareon J, Tokuhisa T
Division of Developmental Genetics, Chiba University School of Medicine, Japan.
Cancer Lett. 1995 Jun 8;92(2):203-8. doi: 10.1016/0304-3835(95)03780-z.
Overexpression of c-Fos/AP-1 augments proliferation of splenic B cells stimulated with lipopolysaccharide (LPS). To elucidate mechanisms of the augmentation by c-Fos/AP-1, a cell cycle of the LPS-activated B cells from c-fos transgenic mice was analyzed. Cell cycle progression into the S phase was accelerated in the c-fos B cells. Expression of genes related to the cell cycle progression was examined in these B cells. Amount of cyclin D3 and cdk4 mRNA increased in the c-fos B cells at 6 h earlier than that in the control B cells, indicating that the kinetics of these mRNA expressions correlate with the acceleration of cell cycle progression. Furthermore, cyclin D1 and cyclin E mRNA were detected in the c-fos B cells but not in the control B cells. These results indicate that deregulated c-Fos/AP-1 modulates expression of the cyclin and the cdk gene in splenic B cells stimulated with LPS. These modulations may accelerate cell cycle progression and augment proliferation of the B cells.
c-Fos/AP-1的过表达增强了用脂多糖（LPS）刺激的脾B细胞的增殖。为了阐明c-Fos/AP-1增强作用的机制，分析了来自c-fos转基因小鼠的LPS激活的B细胞的细胞周期。c-fos B细胞进入S期的细胞周期进程加快。在这些B细胞中检测了与细胞周期进程相关的基因表达。c-fos B细胞中细胞周期蛋白D3和细胞周期蛋白依赖性激酶4（cdk4）mRNA的量比对照B细胞早6小时增加，表明这些mRNA表达的动力学与细胞周期进程的加速相关。此外，在c-fos B细胞中检测到细胞周期蛋白D1和细胞周期蛋白E mRNA，而在对照B细胞中未检测到。这些结果表明，失调的c-Fos/AP-1调节LPS刺激的脾B细胞中细胞周期蛋白和cdk基因的表达。这些调节可能加速细胞周期进程并增强B细胞的增殖。
