Takada M, Koizumi T, Bachiller D, Rüther U, Tokuhisa T
Department of Immunology, ICMR, Kobe University School of Medicine, Hyogo, Japan.
Cancer Lett. 1993 Feb;68(2-3):243-7. doi: 10.1016/0304-3835(93)90153-z.
We have examined effects of the deregulated c-fos protein on the lipopolysaccharide (LPS)-mediated B cell responses using splenic B cells from transgenic lines carrying the mouse c-fos gene under the control of the H-2K (H2-c-fos) and the inducible Mx promoter (Mx-c-fosD). High c-fos expression was induced in Mx-c-fosD B cells by LPS stimulation. DNA synthesis of the B cells from both lines was augmented depending on the amount of exogenous c-fos. This augmentation resulted in the increase of IgM and IgG2b productions in the culture. These results suggest a functional role of c-fos protein in cell cycle progression of the activated B cells.
我们使用了携带在H-2K（H2-c-fos）和可诱导的Mx启动子（Mx-c-fosD）控制下的小鼠c-fos基因的转基因品系的脾B细胞，研究了失调的c-fos蛋白对脂多糖（LPS）介导的B细胞反应的影响。通过LPS刺激在Mx-c-fosD B细胞中诱导了高c-fos表达。两个品系的B细胞的DNA合成根据外源性c-fos的量而增加。这种增加导致培养物中IgM和IgG2b产生的增加。这些结果表明c-fos蛋白在活化B细胞的细胞周期进程中具有功能性作用。
