Phuchareon J, Tokuhisa T
Division of Developmental Genetics, Chiba University School of Medicine, Japan.
Immunobiology. 1995 Aug;193(5):391-9. doi: 10.1016/S0171-2985(11)80426-2.
We implicated deregulated c-Fos/AP-1 in proliferative response of B lymphocytes stimulated with lipopolysaccharide (LPS) using splenic B cells from c-fos transgenic (Mx-c-fos) mice. Levels of DNA synthesis of the Mx-c-fos B cells were augmented in proportion to the amount of AP-1. Since the number of LPS-responding splenic B cells from Mx-c-fos mice was similar to that from control mice duplication time of the Mx-c-fos B cells (0.9 days) was much shorter than that of the control B cells (2.1 days), this augmentation is explained by the acceleration of cell cycle progression by deregulated c-Fos/AP-1. These results suggest that AP-1 is a major regulatory factor for cell cycle progression of B cells activated with LPS.
我们使用来自c-fos转基因（Mx-c-fos）小鼠的脾B细胞，发现c-Fos/AP-1失调与脂多糖（LPS）刺激的B淋巴细胞增殖反应有关。Mx-c-fos B细胞的DNA合成水平与AP-1的量成比例增加。由于Mx-c-fos小鼠对LPS有反应的脾B细胞数量与对照小鼠相似，Mx-c-fos B细胞的复制时间（0.9天）比对照B细胞（2.1天）短得多，这种增加可以用失调的c-Fos/AP-1加速细胞周期进程来解释。这些结果表明，AP-1是LPS激活的B细胞细胞周期进程的主要调节因子。
