Kuramoto H, Endo Y
Department of Applied Biology, Kyoto Institute of Technology, Japan.
Neurosci Lett. 1995 Mar 31;188(3):171-4. doi: 10.1016/0304-3940(95)11424-u.
Galanin (GAL) immunohistochemistry combined with acetylcholinesterase (AChE) histochemistry was applied to demonstrate the innervation of the rat esophageal muscle coats. GAL immunoreactivity was found in a number of nerve cell bodies in the myenteric ganglia and in numerous varicose and non-varicose nerve fibers in the myenteric plexus and around blood vessels. Many GAL-positive varicose fibers ran in the internodal strands and along the striated muscle fibers. They often ramified and terminated on the muscle fibers to form arborizing structures, which were most abundant in the thoracic portion of the esophagus. Such GAL-positive terminals were localized in most (87.7%) of AChE-reactive motor endplates on the esophageal striated muscles. Left supranodose vagotomy caused a significant decrease of the GAL-arborizing terminals on the striated muscles of the esophagus. This suggests that they are terminals of efferent fibers in the vagus nerve.
采用甘丙肽（GAL）免疫组织化学与乙酰胆碱酯酶（AChE）组织化学相结合的方法，以显示大鼠食管肌层的神经支配。在肌间神经节的许多神经细胞体以及肌间神经丛和血管周围的大量曲张和非曲张神经纤维中发现了GAL免疫反应性。许多GAL阳性曲张纤维走行于节间束并沿着横纹肌纤维分布。它们常分支并终止于肌纤维上，形成树枝状结构，在食管胸部最为丰富。这种GAL阳性终末位于食管横纹肌上大多数（87.7%）AChE反应性运动终板处。左迷走神经结上切断术导致食管横纹肌上GAL树枝状终末显著减少。这表明它们是迷走神经传出纤维的终末。
