Wörl J, Fischer J, Neuhuber W L
Anatomy Institute, University of Erlangen-Nürnberg, Krankenhausstrasse 9, D-91054 Erlangen, Germany.
Cell Tissue Res. 1998 Jun;292(3):453-61. doi: 10.1007/s004410051074.
We investigated the origin of galanin-positive nerve fibers on motor endplates in rat esophagus using anterograde 1,1'-dioleyl-3,3,3', 3'-tetramethylindocarbocyanine methane sulfonate (DiI) tracing from the nucleus ambiguus combined with galanin immunocytochemistry and calcitonin gene-related peptide immunocytochemistry. To demonstrate spatial relationships of galanin-positive nerve fibers to vagal and enteric nerve fibers on motor endplates, we combined galanin immunocytochemistry with calcitonin gene-related peptide immunostaining for labeling of vagal terminals, and vasoactive intestinal peptide immunoreactivity and NADPH-diaphorase histochemistry for demonstration of enteric nerve fibers. Within fine varicose nerve fibers, galanin was colocalized with vasoactive intestinal peptide and NADPH-diaphorase to a high degree and turned out to be completely separated from calcitonin gene-related peptide-positive or anterogradely DiI-labeled vagal motor terminals. These results indicate that the enteric nervous system is the most important and possibly the only source of galanin-positive nerve terminals on motor endplates in rat esophagus. Galanin may be, in addition to nitric oxide and vasoactive intestinal peptide, a mediator of the enteric coinnervation of striated muscle in this organ.
我们使用从疑核进行的顺行性1,1'-二油酰基-3,3,3',3'-四甲基吲哚碳菁甲烷磺酸盐（DiI）示踪，结合甘丙肽免疫细胞化学和降钙素基因相关肽免疫细胞化学，研究了大鼠食管运动终板上甘丙肽阳性神经纤维的起源。为了证明运动终板上甘丙肽阳性神经纤维与迷走神经和肠神经纤维的空间关系，我们将甘丙肽免疫细胞化学与降钙素基因相关肽免疫染色相结合以标记迷走神经终末，并将血管活性肠肽免疫反应性和NADPH-黄递酶组织化学相结合以显示肠神经纤维。在细小的曲张神经纤维内，甘丙肽与血管活性肠肽和NADPH-黄递酶高度共定位，并且完全与降钙素基因相关肽阳性或顺行性DiI标记的迷走运动终末分离。这些结果表明，肠神经系统是大鼠食管运动终板上甘丙肽阳性神经终末的最重要且可能是唯一来源。除一氧化氮和血管活性肠肽外，甘丙肽可能是该器官横纹肌肠共同神经支配的一种介质。
