Kuramoto H, Kawano H, Sakamoto H, Furness J B
Department of Applied Biology, Kyoto Institute of Technology, Kyoto 606-8585, Japan.
Cell Tissue Res. 1999 Feb;295(2):241-5. doi: 10.1007/s004410051230.
The relationship between nitric oxide synthase (NOS)- and galanin-immunoreactive nerve terminals and the origin of NOS-immunoreactive nerve terminals on the motor endplates in the striated muscles of the rat esophagus was investigated. Double immunohistochemical staining revealed a dual innervation of motor endplates by calcitonin gene-related peptide (CGRP)-immunoreactive axons and by axons that were immunoreactive for both NOS and galanin. On average, 91% of NOS terminals were galanin immunoreactive. NOS-immunoreactive fibers were revealed at 67% of endplates, identified by the presence of CGRP terminals. The left vagus and superior laryngeal nerve were cut and 15 days allowed for terminals to degenerate. This caused a significant loss of CGRP fibers, but did not affect the density of innervation of the striated muscle by NOS-immunoreactive fibers. Thus the NOS/galanin fibers are deduced to originate from ganglia in the esophageal wall. This is supported by our observation of numerous NOS-immunoreactive nerve cell bodies in the myenteric ganglia of the esophagus, 74% of which were galanin immunoreactive. There were no CGRP-immunoreactive nerve cell bodies in the wall of the esophagus.
研究了大鼠食管横纹肌运动终板上一氧化氮合酶（NOS）和甘丙肽免疫反应性神经末梢之间的关系以及NOS免疫反应性神经末梢的起源。双重免疫组织化学染色显示，降钙素基因相关肽（CGRP）免疫反应性轴突以及对NOS和甘丙肽均呈免疫反应性的轴突对运动终板进行双重支配。平均而言，91%的NOS终末呈甘丙肽免疫反应性。通过CGRP终末的存在鉴定出，67%的终板有NOS免疫反应性纤维。切断左侧迷走神经和喉上神经，并让终末退变15天。这导致CGRP纤维显著减少，但不影响NOS免疫反应性纤维对横纹肌的支配密度。因此，推测NOS/甘丙肽纤维起源于食管壁内的神经节。我们在食管肌间神经节中观察到大量NOS免疫反应性神经细胞体，其中74%呈甘丙肽免疫反应性，这支持了上述推测。食管壁内没有CGRP免疫反应性神经细胞体。
