Anderson C R, Furness J B, Woodman H L, Edwards S L, Crack P J, Smith A I
Department of Anatomy and Cell Biology, University of Melbourne, Parkville, Victoria, Australia.
J Auton Nerv Syst. 1995 Apr 8;52(2-3):107-16. doi: 10.1016/0165-1838(94)00150-i.
Retrograde dye tracing was combined with immunohistochemistry to determine the distributions of nitric oxide synthase (NOS) immunoreactive nerve cells that project to prevertebral ganglia from the gastrointestinal tract and spinal cord of the guinea pig. An antiserum was raised against the neuronal form of NOS by selecting an amino-acid sequence specific to this form as immunogen. The antiserum recognised a single band at 150 kDa on Western blots of rat brain extract. Enteric nerve cells that were labelled by Fast Blue injected into the coeliac ganglion were not NOS immunoreactive in the small intestine, whereas 40-70% were reactive in the large intestine. Retrograde dye injected into the inferior mesenteric ganglion labels cells in the colon and rectum; 60-70% were immunoreactive for NOS. The NOS-immunoreactive nerve fibres arising in the intestine appear to end selectively around somatostatin-immunoreactive nerve cells in the coeliac and inferior mesenteric ganglia. Preganglionic nerve cell bodies in the intermediolateral column and dorsal commissural nucleus from T12 to L2 were labelled from the inferior mesenteric ganglion. Nearly 70% of neurons at each level were NOS immunoreactive. Thus, two sources of NOS terminals in prevertebral ganglia have been identified, intestinofugal neurons of the large, but not the small intestine, and sympathetic preganglionic neurons.
采用逆行染料追踪法并结合免疫组织化学方法，以确定豚鼠胃肠道和脊髓中投射至椎前神经节的一氧化氮合酶（NOS）免疫反应性神经细胞的分布。通过选择该形式特异的氨基酸序列作为免疫原，制备了针对神经元型NOS的抗血清。该抗血清在大鼠脑提取物的蛋白质免疫印迹上识别出一条150 kDa的单一条带。注射到腹腔神经节的快蓝标记的肠神经细胞在小肠中无NOS免疫反应性，而在大肠中有40%-70%呈反应性。注射到肠系膜下神经节的逆行染料标记结肠和直肠中的细胞；60%-70%对NOS呈免疫反应性。肠内产生的NOS免疫反应性神经纤维似乎选择性地终止于腹腔和肠系膜下神经节中生长抑素免疫反应性神经细胞周围。来自肠系膜下神经节的标记显示，T12至L2节段中间外侧柱和背连合核中的节前神经细胞体。每个节段近70%的神经元呈NOS免疫反应性。因此，已确定椎前神经节中NOS终末的两个来源，即大肠而非小肠的肠传出神经元和交感节前神经元。
