Wong L, Oakley B, Lawton A, Shiba Y
Department of Biology, University of Michigan, Ann Arbor 48109.
Chem Senses. 1994 Jun;19(3):251-64. doi: 10.1093/chemse/19.3.251.
Three monoclonal antibodies, 4.62, LP2K and 170.2.14, were used to evaluate keratin 19-like immunoreactivity in gustatory epithelia. Keratin 19-like immunoreactivity was restricted to the intragemmal cells for all types of mammalian taste buds examined. These taste buds included fungiform, foliate and vallate taste buds in rat, gerbil and rabbit, and nasopalatine, epiglottal and palatine taste buds in rat. There was no keratin 19-like immunoreactivity in basal cells or in perigemmal cells lateral to the immunoreactive taste receptor cells. Denervation of the rat vallate papilla eliminated all taste buds, as well as all immunoreactive taste cells. That the immunoreactive material in the taste cells was keratin 19 was supported by the comparable staining of rat taste buds with each of three monoclonal antibodies specific for keratin 19. Furthermore, as predicted, these antibodies selectively stained luminal cells of rat bile ducts, bladder, salivary ducts, trachea, ureter and uterus. It was concluded that monoclonal antibodies against keratin 19 can usefully distinguish intragemmal taste receptor cells from keratinocytes, and from the perigemmal and basal cells of gustatory epithelia. Anti-keratin 19 antibodies may serve to identify differentiated taste cells in gustatory epithelia undergoing taste bud development, renewal, degeneration or regeneration.
使用三种单克隆抗体4.62、LP2K和170.2.14评估味觉上皮中角蛋白19样免疫反应性。在所检查的所有类型哺乳动物味蕾中，角蛋白19样免疫反应性仅限于味蕾内细胞。这些味蕾包括大鼠、沙鼠和兔的菌状、叶状和轮廓乳头味蕾，以及大鼠的鼻腭、会厌和腭味蕾。在基底细胞或免疫反应性味觉受体细胞外侧的味蕾周围细胞中未发现角蛋白19样免疫反应性。大鼠轮廓乳头去神经支配消除了所有味蕾以及所有免疫反应性味觉细胞。三种对角蛋白19具有特异性的单克隆抗体对大鼠味蕾的染色结果相似，这支持了味觉细胞中的免疫反应物质是角蛋白19的观点。此外，正如所预测的，这些抗体选择性地染色大鼠胆管、膀胱、唾液腺导管、气管、输尿管和子宫的管腔细胞。得出的结论是，抗角蛋白19单克隆抗体可有效地区分味蕾内味觉受体细胞与角质形成细胞，以及味觉上皮的味蕾周围细胞和基底细胞。抗角蛋白19抗体可用于识别味觉上皮中正在经历味蕾发育、更新、退化或再生的分化味觉细胞。
