将癌和神经母细胞瘤的转化基因导入小鼠成纤维细胞。

Transforming genes of carcinomas and neuroblastomas introduced into mouse fibroblasts.

作者信息

Shih C, Padhy L C, Murray M, Weinberg R A

出版信息

Nature. 1981 Mar 19;290(5803):261-4. doi: 10.1038/290261a0.

PMID:7207618

Abstract

We have previously demonstrated that DNA of mouse fibroblasts transformed by 3-methylcholanthrene (3-MC) induced foci of transformed cells when applied to monolayer cultures of NIH3T3 cells, which indicates that at least a part of this phenotype is encoded in DNA sequences. However, our conclusions were confined to the effects of DNAs of 3-MC-transformed mouse fibroblasts on recipient NIH3T3 cells, also of mouse fibroblast origin. To elucidate this phenomenon further, we have prepared DNAs from a series of mouse and non-mouse tumour lines of non-fibroblastic origin and investigated whether tumour transforming genes can act across tissue and species barriers to transform NIH3T3 cells. We find that DNAs obtained from human, rabbit and mouse bladder carcinoma lines, a lung carcinoma line and rat neuroblastoma and mouse glioma lines, are able to induce transformation of NIH3T3 cells on transfection.

摘要

我们之前已经证明，用3-甲基胆蒽（3-MC）转化的小鼠成纤维细胞的DNA，应用于NIH3T3细胞的单层培养物时可诱导转化细胞灶形成，这表明该表型的至少一部分由DNA序列编码。然而，我们的结论仅限于3-MC转化的小鼠成纤维细胞的DNA对同样源自小鼠成纤维细胞的受体NIH3T3细胞的影响。为了进一步阐明这一现象，我们从一系列非成纤维细胞起源的小鼠和非小鼠肿瘤细胞系中制备了DNA，并研究肿瘤转化基因是否能够跨越组织和物种屏障来转化NIH3T3细胞。我们发现，从人、兔和小鼠膀胱癌系、一个肺癌系、大鼠神经母细胞瘤和小鼠胶质瘤系获得的DNA，在转染时能够诱导NIH3T3细胞转化。