Jahr T G, Sundan A, Lichenstein H S, Espevik T
Institute of Cancer Research and Molecular Biology, University of Trondheim, Norway.
Scand J Immunol. 1995 Jul;42(1):119-27. doi: 10.1111/j.1365-3083.1995.tb03634.x.
In this study we examined the involvement of human serum, recombinant lipopolysaccharide binding protein (rLBP), recombinant (r)CD14, CD14 antibodies and recombinant bactericidal permeability-increasing factor (rBPI) in the induction of TNF by Salmonella minnesota LPS of different polysaccharide chain lengths. Soluble rCD14 and rLBP markedly enhanced LPS 6261 TNF production and to a lesser degree also enhanced TNF production from Re 595 LPS and lipid A DP. Addition of anti-CD14 antibodies resulted in nearly complete inhibition of LPS 6261-induced TNF production and partial inhibition of Re 595 LPS and lipid A DP-induced TNF release. The ability of lipid A MP to induce TNF production increased with addition of rCD14. Addition of rLBP or anti-CD14 antibodies had no detectable effect on lipid A MP-induced TNF production. The effect of rBPI was also tested and the results showed that only the TNF-inducing ability from smooth LPS was completely inhibited by rBPI. Recombinant BPI was considerably less effective in inhibiting Re 595 LPS-induced TNF production, and lipid A DP was not affected by rBPI. Our data suggest that the ability of rLBP, rCD14, CD14 antibodies and rBPI to modulate LPS induced TNF production is strongly dependent on the LPS polysaccharide chain length.
在本研究中，我们检测了人血清、重组脂多糖结合蛋白（rLBP）、重组（r）CD14、CD14抗体以及重组杀菌通透性增加因子（rBPI）在不同多糖链长度的明尼苏达沙门氏菌脂多糖（LPS）诱导肿瘤坏死因子（TNF）过程中的作用。可溶性rCD14和rLBP显著增强了LPS 6261诱导的TNF产生，在较小程度上也增强了Re 595 LPS和脂质A DP诱导的TNF产生。添加抗CD14抗体几乎完全抑制了LPS 6261诱导的TNF产生，并部分抑制了Re 595 LPS和脂质A DP诱导的TNF释放。随着rCD14的添加，脂质A MP诱导TNF产生的能力增强。添加rLBP或抗CD14抗体对脂质A MP诱导的TNF产生没有可检测到的影响。我们还测试了rBPI的作用，结果表明只有光滑型LPS诱导TNF产生的能力被rBPI完全抑制。重组BPI在抑制Re 595 LPS诱导的TNF产生方面效果显著降低，脂质A DP不受rBPI影响。我们的数据表明，rLBP、rCD14、CD14抗体和rBPI调节LPS诱导TNF产生的能力强烈依赖于LPS的多糖链长度。
