Kasik J W, Rice E J
Department of Pediatrics, Metrohealth Medical Center, Case Western Reserve University, Cleveland, OH 44109, USA.
Am J Obstet Gynecol. 1995 Aug;173(2):613-7. doi: 10.1016/0002-9378(95)90291-0.
To provide novel insights into the molecular events associated with parturition, a differential screen was made of a mouse uterine complementary deoxyribonucleic acid library to identify selectively expressed genes in late pregnancy.
A differential hybridization was used to screen a mouse complementary deoxyribonucleic acid library prepared from late pregnancy uterus. A 1 kb clone was isolated that was subsequently identified as 24p3, a member of the lipocalin family. By use of radiolabeled complementary deoxyribonucleic acid probes prepared from this clone Northern hybridizations were conducted against total ribonucleic acid purified from mouse uterus collected during late pregnancy and the first week after birth and a variety of other mouse tissues to determine whether this gene is selectively expressed in the uterus coincident with parturition.
Low levels of message for the 24p3 gene could be detected in uterine ribonucleic acid, but there was a massive increase in the level of message for this gene on the days surrounding birth. Northern hybridizations conducted against additional tissues collected from both pregnant and nonpregnant mice did not detect message to a similar degree as found in the uterus.
The uterus constitutes a major site of expression of this gene, particularly near birth. The expression of this gene coincident with birth suggests a potential physiologic role of the neutrophil with the induction of labor.
为深入了解与分娩相关的分子事件，对小鼠子宫互补脱氧核糖核酸文库进行差异筛选，以鉴定妊娠晚期选择性表达的基因。
采用差异杂交技术筛选从妊娠晚期子宫制备的小鼠互补脱氧核糖核酸文库。分离出一个1 kb的克隆，随后鉴定为24p3，属于脂质运载蛋白家族成员。利用从该克隆制备的放射性标记互补脱氧核糖核酸探针，对从妊娠晚期及出生后第一周收集的小鼠子宫纯化的总核糖核酸以及多种其他小鼠组织进行Northern杂交，以确定该基因是否在分娩时子宫中选择性表达。
在子宫核糖核酸中可检测到低水平的24p3基因信息，但在出生前后几天该基因的信息水平大幅增加。对从妊娠和未妊娠小鼠收集的其他组织进行的Northern杂交未检测到与子宫中相似程度的信息。
子宫是该基因表达的主要部位，尤其是在临近出生时。该基因在出生时的表达表明嗜中性粒细胞在分娩诱导中可能具有生理作用。
