Kasik J, Rice E
Metrohealth Medical Center, Case Western Reserve University, Cleveland, OH 44109, USA.
Am J Obstet Gynecol. 1997 Feb;176(2):452-6. doi: 10.1016/s0002-9378(97)70514-3.
Our purpose was to identify novel genes expressed by the uterus during late pregnancy.
A complementary deoxyribonucleic acid library constructed from late pregnancy mouse uterus was screened by differential hybridization with complementary deoxyribonucleic acid probes constructed from late pregnancy mouse uterus and nonpregnant mouse uterus. Radiolabeled complementary deoxyribonucleic acid probes derived from one of the complementary deoxyribonucleic acids isolated were used in northern hybridizations against ribonucleic acid collected from pregnant and nonpregnant uterus and a variety of other mouse tissues.
A total of 40 positive clones were isolated; half were identified as cytotoxic T-lymphocyte antigen-2 alpha (a putative inhibitor of the protease cathepsin L) and the other half represented a novel complementary deoxyribonucleic acid. Conceptual translation of the complementary deoxyribonucleic acid predicted a novel protein of 154 amino acids that is proline rich and acidic (pregnancy-specific uterine protein). Northern hybridizations demonstrated that message is abundant in the uterus during late pregnancy. After birth expression rapidly decreased and message was no longer found in the uterus by the third day. A minimal amount of message is present in placental ribonucleic acid, but expression is otherwise not detected in a variety of adult and fetal tissues surveyed, suggesting that expression of this gene is limited to the pregnant uterus.
The abundance of message and expression apparently limited to the pregnant uterus suggests that the protein represented by this complementary deoxyribonucleic acid may play an important role in pregnancy.
我们的目的是鉴定妊娠晚期子宫所表达的新基因。
用从妊娠晚期小鼠子宫构建的互补脱氧核糖核酸探针与从妊娠晚期小鼠子宫和未孕小鼠子宫构建的互补脱氧核糖核酸探针进行差异杂交，筛选从妊娠晚期小鼠子宫构建的互补脱氧核糖核酸文库。从分离出的一种互补脱氧核糖核酸衍生的放射性标记互补脱氧核糖核酸探针用于对从妊娠和未孕子宫以及多种其他小鼠组织收集的核糖核酸进行Northern杂交。
共分离出40个阳性克隆；其中一半被鉴定为细胞毒性T淋巴细胞抗原-2α（一种假定的蛋白酶组织蛋白酶L抑制剂），另一半代表一种新的互补脱氧核糖核酸。该互补脱氧核糖核酸的概念性翻译预测了一种由154个氨基酸组成的新蛋白质，富含脯氨酸且呈酸性（妊娠特异性子宫蛋白）。Northern杂交表明，该信息在妊娠晚期子宫中丰富。出生后表达迅速下降，到第三天子宫中不再能检测到该信息。胎盘核糖核酸中存在少量该信息，但在检测的多种成年和胎儿组织中未检测到其他表达，表明该基因的表达仅限于妊娠子宫。
该信息丰富且表达明显仅限于妊娠子宫，提示这种互补脱氧核糖核酸所代表的蛋白质可能在妊娠中起重要作用。
