Seitz S E, Foley G L, Marretta S M
Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Urbana 61801, USA.
Am J Vet Res. 1995 Jun;56(6):826-33.
Microscopic evaluation of the margins of excised cutaneous neoplasms is of paramount importance for determining that neoplastic tissue does not extend to the excision borders. Dyes or pigments that indelibly mark the tissue should be more reliable than sectioning techniques or suture markers for identifying the surgical margins before and after tissue processing. We evaluated 5 pigments to select a material that could be rapidly applied by surgeons, and readily identified on histologic section by the pathologists. Twenty normal canine skin specimens were assigned to each of 5 groups. Each group was treated with artists' pigments in acetone, India ink in acetone, alcian blue, typists' correction fluid, or a commercially available marking kit. Ten specimens within each group were marked before formalin fixation, and 10 were marked after fixation. Application properties, fixation and processing properties, and microscopic characteristics were evaluated for each material. Application properties were acceptable for all marking materials on unfixed specimens, and for alcian blue, India ink in acetone, and correction fluid on fixed specimens. Fixation and processing properties were acceptable for all materials except correction fluid. All marking materials survived fixation and processing, and colors were readily visualized under the microscope. Microscopic characteristics were acceptable for alcian blue, India ink in acetone, and the commercial kit. Overall, alcian blue was the best marking material, with India ink in acetone and the commercial kit also acceptable. Correction fluid and artist' pigments in acetone were not acceptable because pigment fragmentation and incomplete tissue coverage hindered microscopic evaluation of resection margins.
对切除的皮肤肿瘤边缘进行显微镜评估对于确定肿瘤组织未延伸至切除边界至关重要。在组织处理前后，用于不可磨灭地标记组织的染料或色素应该比切片技术或缝线标记物在识别手术切缘方面更可靠。我们评估了5种色素，以选择一种外科医生能够快速应用且病理学家在组织切片上易于识别的材料。将20个正常犬皮肤标本分配到5组中的每组。每组分别用丙酮中的美术颜料、丙酮中的印度墨水、阿尔辛蓝、打字员修正液或市售标记试剂盒进行处理。每组中的10个标本在福尔马林固定前进行标记，10个在固定后进行标记。对每种材料的应用特性、固定和处理特性以及微观特征进行了评估。所有标记材料在未固定标本上的应用特性均可接受，阿尔辛蓝、丙酮中的印度墨水和修正液在固定标本上的应用特性也可接受。除修正液外，所有材料的固定和处理特性均可接受。所有标记材料在固定和处理后均留存，并且在显微镜下颜色易于观察。阿尔辛蓝、丙酮中的印度墨水和市售试剂盒的微观特征均可接受。总体而言，阿尔辛蓝是最佳标记材料，丙酮中的印度墨水和市售试剂盒也可接受。修正液和丙酮中的美术颜料不可接受，因为色素碎片和不完全的组织覆盖妨碍了对切除边缘的显微镜评估。
