Rigler R
Department of Medical Biophysics, Karolinska Institute, Stockholm, Sweden.
J Biotechnol. 1995 Jul 31;41(2-3):177-86. doi: 10.1016/0168-1656(95)00054-t.
Fluorescence correlation spectroscopy (FCS), when carried out under conditions with low background as obtained in very small volume elements, is a powerful tool for examining molecular interactions as well as their time dependence. Interactions of biological importance which can be analyzed are hybridization between nucleic acid primers and DNA or RNA targets, between peptide ligands and isolated as well as cell-bound receptors, between antigen and antibodies. Since the interaction can be analyzed rapidly in small volumes without the need for separating unbound from bound ligand, an important application of FCS is envisaged in large-scale drug screening. The sensitivity has been advanced to the point that detection of single dye molecules is possible in the submillisecond range. This opens up the possibility for detecting rare events such as the appearance of pathogens in the early phase of infection or mutants exhibiting unusual properties when screening combinatorial libraries.
荧光相关光谱法（FCS），在诸如非常小的体积元中获得的低背景条件下进行时，是一种用于研究分子相互作用及其时间依赖性的强大工具。可以分析的具有生物学重要性的相互作用包括核酸引物与DNA或RNA靶标之间、肽配体与分离的以及细胞结合的受体之间、抗原与抗体之间的杂交。由于无需将未结合的配体与结合的配体分离，就能在小体积中快速分析相互作用，因此FCS在大规模药物筛选中具有重要应用前景。其灵敏度已提高到在亚毫秒范围内能够检测单个染料分子的程度。这为检测罕见事件开辟了可能性，例如在感染早期病原体的出现，或在筛选组合文库时表现出异常特性的突变体。
