Eigen M, Rigler R
Max-Planck-Institut für Biophysikalische Chemie, Göttingen, Federal Republic of Germany.
Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):5740-7. doi: 10.1073/pnas.91.13.5740.
A method is described that provides for detection and identification of single molecules in solution. The method is based on fluorescence correlation spectroscopy, which records spatio-temporal correlations among fluctuating light signals, coupled with devices for trapping single molecules in an electric field. This technique is applied to studies of molecular evolution, where it allows fast screening of large mutant spectra in which targets are labeled by specific fluorescent ligands. The method expands the horizon in molecular diagnostics by making it possible to monitor concentrations down to (less than) 10(-15) M without any need for amplification.
本文描述了一种用于检测和识别溶液中单分子的方法。该方法基于荧光相关光谱技术，它记录波动光信号之间的时空相关性，并结合用于在电场中捕获单分子的装置。这项技术应用于分子进化研究，能够快速筛选大型突变谱，其中目标由特定荧光配体标记。该方法通过能够监测低至（小于）10^(-15) M的浓度而无需任何扩增，拓展了分子诊断的视野。
