Michaëlsson E, Holmdahl M, Engström A, Burkhardt H, Scheynius A, Holmdahl R
Department of Cellular and Molecular Biology, Lund University, Sweden.
Eur J Immunol. 1995 Aug;25(8):2234-41. doi: 10.1002/eji.1830250818.
Dendritic cells, such as epidermal Langerhans cells, play a crucial role for the antigen-specific priming of T cells. We have addressed the question whether dendritic cells present collagen, a major protein component in tissues through which dendritic cells migrate, i.e. the basement membrane, dermis, and synovial tissue. Langerhans cells, spleen cells and peritoneal macrophages were compared for antigen-presenting capacity using a panel of mouse T cell hybridomas reactive with different determinants on type II collagen, myelin basic protein, ovalbumin and pepsin. Langerhans cells did not present any of the type II collagen determinants, unless the antigen was administered as a 15-mer peptide, but did present myelin basic protein, ovalbumin and pepsin. Spleen cells and peritoneal macrophages, in contrast, presented all type II collagen determinants. This biased antigen presentation was also observed when Langerhans cells were pulsed with antigen in vivo. The inability to present type II collagen is related to the collagen sequence as such, since both native type II collagen, type II collagen alpha chains, as well as a type II collagen determinant incorporated in type I collagen, were not presented by Langerhans cells. In addition, granulocyte/macrophage colony-stimulating factor-expanded blood dendritic cells displayed the same biased antigen presentation, suggesting that the inability to present collagen is not restricted to dendritic cells localized in epidermis. B cell-deficient mice could prime a type II collagen-reactive T cell response, thus excluding B cells as obligatory antigen-presenting cells for the priming of collagen-reactive T cells. We suggest that neither Langerhans cells nor B cells, but macrophages are the primary antigen-presenting cells in the immune response towards type II collagen.
树突状细胞，如表皮朗格汉斯细胞，在T细胞的抗原特异性启动中起关键作用。我们探讨了树突状细胞是否呈递胶原蛋白的问题，胶原蛋白是树突状细胞迁移所经过的组织中的一种主要蛋白质成分，即基底膜、真皮和滑膜组织。使用一组与II型胶原蛋白、髓鞘碱性蛋白、卵清蛋白和胃蛋白酶上不同决定簇反应的小鼠T细胞杂交瘤，比较了朗格汉斯细胞、脾细胞和腹腔巨噬细胞的抗原呈递能力。朗格汉斯细胞不呈递任何II型胶原蛋白决定簇，除非抗原以15聚体肽的形式给药，但能呈递髓鞘碱性蛋白、卵清蛋白和胃蛋白酶。相比之下，脾细胞和腹腔巨噬细胞呈递所有II型胶原蛋白决定簇。当朗格汉斯细胞在体内用抗原脉冲时，也观察到这种有偏向性的抗原呈递。无法呈递II型胶原蛋白与胶原蛋白序列本身有关，因为天然II型胶原蛋白、II型胶原蛋白α链以及掺入I型胶原蛋白中的II型胶原蛋白决定簇，朗格汉斯细胞均不呈递。此外，粒细胞/巨噬细胞集落刺激因子扩增的血液树突状细胞也表现出相同的有偏向性的抗原呈递，这表明无法呈递胶原蛋白并不局限于表皮中的树突状细胞。B细胞缺陷小鼠可以启动II型胶原蛋白反应性T细胞应答，因此排除了B细胞作为启动胶原蛋白反应性T细胞的必需抗原呈递细胞。我们认为，在针对II型胶原蛋白的免疫应答中，朗格汉斯细胞和B细胞都不是主要的抗原呈递细胞，巨噬细胞才是。
