Haase A, Melder A, Smith-Vaughan H, Kemp D, Currie B
Menzies School of Health Research, Casuarina, Australia.
Epidemiol Infect. 1995 Aug;115(1):115-21. doi: 10.1017/s0950268800058179.
Twenty-seven isolates of Burholderia pseudomallei (formerly Pseudomonas pseudomallei) from ten patients with recurrent melioidosis were analysed by RAPD. In two cases RAPD patterns in recurrent isolates differed from the original isolates; one was considered a likely reinfection while the other may represent relapse from one of two strains initially infecting the patient. In two cases where a change in antibiotic resistance had occurred between original and relapse isolates, slight changes in RAPD patterns were found with one of the four primers used. In the other six cases the relapse was clearly due to the original strain re-emerging unchanged, with identical RAPD patterns with all four primers.
对来自10例反复发生类鼻疽病患者的27株类鼻疽伯克霍尔德菌（以前称为类鼻疽假单胞菌）进行了随机扩增多态性DNA（RAPD）分析。在2例中，反复分离株的RAPD图谱与原始分离株不同；1例被认为可能是再次感染，而另1例可能代表最初感染患者的两种菌株之一复发。在2例原始分离株和复发分离株之间出现抗生素耐药性变化的病例中，使用的4种引物之一检测到RAPD图谱有轻微变化。在其他6例中，复发显然是由于原始菌株未发生变化而再次出现，所有4种引物的RAPD图谱均相同。
