Lew A E, Desmarchelier P M
Department of Microbiology, University of Queensland, St. Lucia, Australia.
J Clin Microbiol. 1993 Mar;31(3):533-9. doi: 10.1128/jcm.31.3.533-539.1993.
The aim of this study was to develop a typing scheme for Pseudomonas pseudomallei by comparison of patterns of restriction fragment length polymorphisms in rRNA genes (ribotyping). BamHI restriction digests of 100 isolates from various animal (34), human (58), and environmental (6) sources, including six reference strains, were hybridized to Escherichia coli 16S and 23S rRNAs. A chemiluminescent labelling and detection system was used to visualize bands. On the basis of patterns, the strains were classified into 22 different groups, with the largest containing 29 isolates. While most of the ribotypes were not exclusive to a particular source, some ribotypes were restricted to a particular geographic area or to either a human or a particular animal species. Application of the typing scheme to isolates of four independent outbreaks among animals showed that certain ribotypes predominated. The study demonstrated ribotyping to be a useful tool in epidemiological investigations of melioidosis.
本研究的目的是通过比较rRNA基因中限制性片段长度多态性模式（核糖体分型）来开发一种针对类鼻疽杆菌的分型方案。对来自各种动物（34株）、人类（58株）和环境（6株）来源的100株分离株（包括6株参考菌株）进行BamHI限制性酶切，然后与大肠杆菌16S和23S rRNA杂交。使用化学发光标记和检测系统来观察条带。根据模式，这些菌株被分为22个不同的组，最大的组包含29株分离株。虽然大多数核糖体分型并非特定来源所独有，但有些核糖体分型局限于特定地理区域或人类或特定动物物种。将该分型方案应用于动物中四次独立暴发的分离株表明，某些核糖体分型占主导地位。该研究证明核糖体分型是类鼻疽病流行病学调查中的一种有用工具。
