Belkowski S M, Zhu J, Liu-Chen L Y, Eisenstein T K, Adler M W, Rogers T J
Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA 19140, USA.
Adv Exp Med Biol. 1995;373:11-6. doi: 10.1007/978-1-4615-1951-5_2.
The R1.1 cell line has been shown to express kappa-opioid receptors on the cell surface. Our analysis shows that the R1.1 cell line exhibits a CD4NEG CD8NEG CD3LOW CD25LOW cell surface phenotype, characteristic of thymocytes in one of the early stages of differentiation. We have developed reverse transcriptase polymerase chain reaction (RT-PCR) conditions that permit the detection of mRNA coding for the kappa-receptor. Using cell fractionation techniques we have isolated CD4NEG CD8NEG thymocytes, and analysis by RT-PCR shows that these primary immature thymocytes also express the kappa-opioid receptor. We hypothesize that the expression of kappa-opioid receptor may be a marker which is characteristic of immature T development.
R1.1细胞系已被证明在细胞表面表达κ-阿片受体。我们的分析表明，R1.1细胞系呈现出CD4阴性、CD8阴性、CD3低表达、CD25低表达的细胞表面表型，这是分化早期阶段胸腺细胞的特征。我们已经开发出逆转录聚合酶链反应（RT-PCR）条件，能够检测编码κ-受体的mRNA。利用细胞分级分离技术，我们分离出了CD4阴性、CD8阴性的胸腺细胞，RT-PCR分析表明这些原代未成熟胸腺细胞也表达κ-阿片受体。我们推测κ-阿片受体的表达可能是未成熟T细胞发育的一个特征性标志物。
