Belkowski S M, Zhu J, Liu-Chen L Y, Eisenstein T K, Adler M W, Rogers T J
Department of Microbiology, Temple University School of Medicine, Philadelphia, PA 19140, USA.
J Neuroimmunol. 1995 Oct;62(1):113-7. doi: 10.1016/0165-5728(95)00116-j.
To our knowledge, this is the first demonstration of kappa-opioid receptor mRNA in cells of the immune system. While the presence of opioid receptors on cells of the immune system has been controversial, cell-binding analysis has indicated that the kappa-opioid receptor is expressed by the immature T cell line R1.1. We have developed a reverse transcriptase-polymerase chain reaction protocol to amplify the mRNA extracted from R1.1 cells with primers derived from the cDNA sequence of the mouse kappa-opioid receptor. Nucleotide sequences of the amplified products were examined and two populations of cDNA were detected which differ in the 5' region upstream of the ATG start codon. Comparison of these sequences to the previously published kappa-opioid receptor cDNA sequence suggests the presence of an intron-exon junction in the 5' non-coding region.
据我们所知，这是首次在免疫系统细胞中证实κ-阿片受体mRNA的存在。虽然免疫系统细胞上存在阿片受体一直存在争议，但细胞结合分析表明，未成熟T细胞系R1.1表达κ-阿片受体。我们开发了一种逆转录聚合酶链反应方案，用源自小鼠κ-阿片受体cDNA序列的引物扩增从R1.1细胞中提取的mRNA。检查扩增产物的核苷酸序列，检测到两个cDNA群体，它们在ATG起始密码子上游的5'区域不同。将这些序列与先前发表的κ-阿片受体cDNA序列进行比较，表明在5'非编码区域存在内含子-外显子连接。
