Chuang L F, Chuang T K, Killam K F, Qiu Q, Wang X R, Lin J J, Kung H F, Sheng W, Chao C, Yu L
Department of Medical Pharmacology and Toxicology, University of California, Davis 95616, USA.
Biochem Biophys Res Commun. 1995 Apr 26;209(3):1003-10. doi: 10.1006/bbrc.1995.1597.
mRNA encoding the kappa opioid receptor gene sequence was identified and isolated from various human lymphocytic cells: CEM x174 (a hybrid of T and B origin) cells, Jurkat-T4 cells, human peripheral blood mononuclear cells (PBMC) and purified CD4+ cells. Analyzing the cDNA sequences of RNA transcripts spanning the putative second extracellular loop, which has reported dynorphin specificity, and the seventh transmembrane domain revealed a 100% and 95% homology in amino acid sequence to corresponding kappa opioid receptor sequences in human placenta and rat brain, respectively. Expression of a similar kappa opioid receptor sequence could be detected in normal monkey PBMC but not in monkey PBMC in which the CD4+/CD8+ cell ratio (or CD4+ cell number) was significantly reduced due to prolonged SIV infection. These findings suggest that human and monkey lymphocytes constitutively express kappa opioid receptor mRNA.
编码κ阿片受体基因序列的信使核糖核酸（mRNA）从各种人类淋巴细胞中被鉴定并分离出来：CEM x174（T和B起源的杂交细胞）、Jurkat - T4细胞、人外周血单个核细胞（PBMC）以及纯化的CD4 +细胞。分析跨越假定的第二个细胞外环（已报道其具有强啡肽特异性）和第七个跨膜结构域的RNA转录本的互补脱氧核糖核酸（cDNA）序列，结果显示，其氨基酸序列与人类胎盘和大鼠脑中相应的κ阿片受体序列分别具有100%和95%的同源性。在正常猴PBMC中可检测到相似的κ阿片受体序列的表达，但在因长期感染猴免疫缺陷病毒（SIV）而导致CD4 + /CD8 +细胞比率（或CD4 +细胞数量）显著降低的猴PBMC中则检测不到。这些发现表明，人类和猴淋巴细胞组成性地表达κ阿片受体mRNA。
