Kelleher D, Murphy A, Hall N, Omary M B, Kearns G, Long A, Casey E B
Department of Clinical Medicine, Trinity College Dublin, Ireland.
Ann Rheum Dis. 1995 Jul;54(7):566-70. doi: 10.1136/ard.54.7.566.
To investigate the involvement of the adhesion molecule CD44 in the homing of lymphocytes to synovial tissue, by examining the density of expression and molecular mass of CD44 on rheumatoid synovial fluid lymphocytes.
Twenty patients with rheumatoid arthritis were studied. Peripheral blood and synovial fluid lymphocytes were isolated by Ficoll-Hypaque sedimentation. CD44 expression was analysed by two colour flow cytometry of CD3 positive T lymphocytes with calculation of mean fluorescence intensity. Expression of activation markers M21C5, M2B3, interleukin (IL)-2 receptor and transferrin receptor was quantitated. In addition, CD44 molecular mass was examined by Western blot in six patients.
CD44 expression was markedly increased on synovial fluid T lymphocytes of rheumatoid patients relative to peripheral blood lymphocytes from the same individuals. CD44 molecular mass on peripheral blood mononuclear cells was 88 kDa, but that on synovial fluid lymphocytes was only 83 kDa. CD44 expression correlated significantly with expression of activation markers M21C5, M2B3, and the IL-2 receptor.
Alterations in density of expression or of the molecular mass of CD44 could contribute to local tissue injury, either directly by facilitating adhesion, or indirectly through effects on other adhesion molecules.
通过检测类风湿性滑液淋巴细胞上CD44的表达密度和分子量，研究黏附分子CD44在淋巴细胞归巢至滑膜组织中的作用。
对20例类风湿性关节炎患者进行研究。采用Ficoll-Hypaque沉降法分离外周血和滑液淋巴细胞。通过对CD3阳性T淋巴细胞进行双色流式细胞术分析CD44表达，并计算平均荧光强度。对活化标志物M21C5、M2B3、白细胞介素（IL）-2受体和转铁蛋白受体的表达进行定量分析。此外，对6例患者采用蛋白质免疫印迹法检测CD44分子量。
与同一患者的外周血淋巴细胞相比，类风湿患者滑液T淋巴细胞上的CD44表达显著增加。外周血单个核细胞上的CD44分子量为88 kDa，而滑液淋巴细胞上的CD44分子量仅为83 kDa。CD44表达与活化标志物M21C5、M2B3及IL-2受体的表达显著相关。
CD44表达密度或分子量的改变可能直接通过促进黏附或间接通过影响其他黏附分子而导致局部组织损伤。
