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Complete RNA editing of unspliced and dicistronic transcripts of the intron-containing reading frame IRF170 from maize chloroplasts.

作者信息

Ruf S, Zeltz P, Kössel H

机构信息

Institut für Biologie III, Universität Freiburg, Germany.

出版信息

Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2295-9. doi: 10.1073/pnas.91.6.2295.

DOI:10.1073/pnas.91.6.2295
PMID:7545915
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC43357/
Abstract

The maize plastome harbors within the rps4-rps14 gene cluster the reading frame IRF170, which is interrupted by two introns. Although the function of the encoded peptide of 170 amino acids is not known, the conservation of IRF170 homologs in other plastomes is a strong indication that IRF170 is a functional gene. Amplification and sequence analyses of IRF170 specific cDNAs reveals two C-to-U editing events occurring within each of the first two exons. This situation allows an analysis of the temporal order between editing and splicing of a chloroplast transcript. By using intron-specific primer combinations, cDNAs derived from partially or even unspliced IRF170 transcripts could be amplified which in all cases showed complete editing. Complete editing was also observed with a cDNA derived from a transcript in which the proximal rps4 and the 5' half of IRF170-encoded sequences were still linked. This demonstrates that editing of the IRF170 transcript is an early processing step preceding both splicing and cleavage to monocistronic mRNA.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc80/43357/e71eceba5343/pnas01128-0326-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc80/43357/c850b003fbd1/pnas01128-0324-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc80/43357/81c3708193f4/pnas01128-0325-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc80/43357/d44476fd4411/pnas01128-0325-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc80/43357/e71eceba5343/pnas01128-0326-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc80/43357/c850b003fbd1/pnas01128-0324-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc80/43357/81c3708193f4/pnas01128-0325-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc80/43357/d44476fd4411/pnas01128-0325-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc80/43357/e71eceba5343/pnas01128-0326-a.jpg

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本文引用的文献

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Plant Physiol. 1992 Oct;100(2):958-64. doi: 10.1104/pp.100.2.958.
2
RNA editing: exploring one mode with apolipoprotein B mRNA.RNA编辑：以载脂蛋白B信使核糖核酸探索一种模式
Bioessays. 1993 Jan;15(1):33-41. doi: 10.1002/bies.950150106.
3
RNA editing in maize chloroplasts is a processing step independent of splicing and cleavage to monocistronic mRNAs.玉米叶绿体中的RNA编辑是一个独立于剪接和切割成单顺反子mRNA的加工步骤。
Gene Regul Syst Bio. 2009 Mar 12;3:31-47. doi: 10.4137/grsb.s2080.
4
A cytoplasmically inherited barley mutant is defective in photosystem I assembly due to a temperature-sensitive defect in ycf3 splicing.一个细胞质遗传的大麦突变体由于 ycf3 剪接的温度敏感缺陷而在光合作用 I 组装中出现缺陷。
Plant Physiol. 2009 Dec;151(4):1802-11. doi: 10.1104/pp.109.147843. Epub 2009 Oct 7.
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RNA editing in ribosome-less plastids of iojap maize.iojap玉米无核糖体质体中的RNA编辑
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Nucleic Acids Res. 2004 Jan 26;32(2):542-50. doi: 10.1093/nar/gkh217. Print 2004.
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RNA editing in hornwort chloroplasts makes more than half the genes functional.角苔叶绿体中的RNA编辑使超过半数的基因具有功能。
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