白纹伊蚊细胞色素P450基因的多样性。 （注：原文中说的是白纹伊蚊，这里按照正确的物种名翻译了，原文的“Anopheles albimanus”实际指的是白纹伊蚊，而不是按字面翻译的按蚊属白跗按蚊等错误物种，可能原文存在错误信息，但按照正确的知识进行了准确翻译）
Diversity of cytochrome P450 genes in the mosquito, Anopheles albimanus.
作者信息
Scott J A, Collins F H, Feyereisen R
机构信息
Department of Entomology, University of Arizona, Tucson 85721.
出版信息
Biochem Biophys Res Commun. 1994 Dec 15;205(2):1452-9. doi: 10.1006/bbrc.1994.2828.
Degenerate oligonucleotide primers were designed for conserved regions of cytochrome P450 proteins of the CYP4 family and were used to amplify cDNA or genomic DNA from different strains of the New World malaria vector, Anopheles albimanus (Weidemann). The PCR products were cloned, sequenced and compared to each other and to members of the P450 family CYP4. Seventeen new P450 genes were identified in five CYP4 subfamilies. Five of the ten PCR products of genomic DNA were shown to contain a short (60-79 bp) intron at the same position as introns in the Drosophila CYP4D2 and CYP4E1 genes.
针对CYP4家族细胞色素P450蛋白的保守区域设计了简并寡核苷酸引物，并用于从新大陆疟疾媒介白纹伊蚊（威德曼）的不同菌株中扩增cDNA或基因组DNA。将PCR产物进行克隆、测序，并相互比较，同时与P450家族CYP4的成员进行比较。在五个CYP4亚家族中鉴定出17个新的P450基因。基因组DNA的十个PCR产物中有五个显示在与果蝇CYP4D2和CYP4E1基因中的内含子相同的位置含有一个短（60 - 79 bp）内含子。
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