Dunkov B C, Rodriguez-Arnaiz R, Pittendrigh B, ffrench-Constant R H, Feyereisen R
Department of Entomology, University of Arizona, Tucson 85721, USA.
Mol Gen Genet. 1996 Jun 12;251(3):290-7. doi: 10.1007/BF02172519.
Twelve cytochrome P450 cDNA fragments were cloned from Drosophila melanogaster by reverse transcriptase/PCR (RT/PCR) using degenerate oligonucleotide primers. The corresponding genes belong to several subfamilies of the CYP4 and CYP9 P450 families. Only two of these genes, Cyp4dl and Cyp4d2, have previously been described. In situ hybridization of each of the cDNA fragments showed two clusters of genes; one near the tip of the X chromosome and the other on the left arm of chromosome 2. Interestingly the latter cluster comprises widely divergent genes belonging both to the CYP9 and CYP4 families and also to the CYP6 family (Cyp6a2). Putative allelic variants of several of the genes were found in different insecticide-resistant and -susceptible strains (Hikone R, Haag 79 and Oregon R). The identification of these genes and alleles will allow us to clarify the involvement of P450s in xenobiotic metabolism and will facilitate a genetic analysis of P450 functions in insects.
使用简并寡核苷酸引物，通过逆转录酶/聚合酶链反应（RT/PCR）从黑腹果蝇中克隆出12个细胞色素P450 cDNA片段。相应的基因属于CYP4和CYP9 P450家族的几个亚家族。这些基因中只有两个，即Cyp4dl和Cyp4d2，此前已有描述。对每个cDNA片段进行原位杂交显示有两个基因簇；一个靠近X染色体的末端，另一个在2号染色体的左臂上。有趣的是，后一个基因簇包含了属于CYP9和CYP4家族以及CYP6家族（Cyp6a2）的差异很大的基因。在不同的抗杀虫剂和敏感品系（彦根R、哈格79和俄勒冈R）中发现了几个基因的推定等位变体。这些基因和等位基因的鉴定将使我们能够阐明P450在异源物质代谢中的作用，并将有助于对昆虫中P450功能进行遗传分析。
