Couto-Fernandez J C, Janssens W, Heyndrickx L, Motte J, Fransen K, Peeters M, Delaporte E, Galvão-Castro B, Piot P, van der Groen G
Advanced Public Health Laboratory, Centro de Pesquisas Gonçalo, Fundação Oswaldo Cruz, Bahia CEP, Brazil.
AIDS Res Hum Retroviruses. 1994 Sep;10(9):1157-63. doi: 10.1089/aid.1994.10.1157.
Six Brazilian strains of human immunodeficiency virus type 1 (HIV-1) were isolated from infected individuals residing in different regions of Brazil between 1987 and 1989. Phylogenetic analysis based on an 860-base pair env fragment, including V3, V4, V5, and the beginning of gp41, classified the Brazilian strains significantly in genotype B, with interhost distances between 5.9 and 13.1% (mean value, 10%). Amino acid sequence analysis of the V3 loop revealed that three strains contained the North American/European GPGR motif as the tip of the loop whereas in the other three strains proline (P) was substituted by tryptophan (W), methionine (M), or phenylalanine (F). A consensus peptide, Bra-cons, was designed containing GWGR as the tip of the loop. Serological reactivity to the Bra-cons peptide and other V3 peptides (MN, SF2, HBX2, RF, MAL, ELI, Z6, and a Côte d'Ivoire peptide, CI-cons) was compared for 114 HIV-1-positive sera from Rio de Janeiro. Sixty-nine sera (60.5%) reacted with peptides belonging to genotype B, of which 10 sera also reacted with peptides belonging to genotype A (n = 7) and D (n = 3). Eighteen sera (15.8%) had binding antibodies to the Bra-cons peptide. A high number of sera (n = 43; 37.7%) had no antibodies to any of the V3 peptides tested. This result suggests that HIV-1 variants with aberrant V3 loops may circulate in Rio de Janeiro.
1987年至1989年间，从居住在巴西不同地区的受感染个体中分离出6株巴西1型人类免疫缺陷病毒（HIV-1）。基于一个860个碱基对的env片段（包括V3、V4、V5和gp41起始部分）进行的系统发育分析，将巴西毒株显著归类为B基因型，宿主间距离在5.9%至13.1%之间（平均值为10%）。V3环的氨基酸序列分析表明，三株毒株在环的顶端含有北美/欧洲的GPGR基序，而在其他三株毒株中，脯氨酸（P）被色氨酸（W）、甲硫氨酸（M）或苯丙氨酸（F）取代。设计了一种共有肽Bra-cons，其环的顶端为GWGR。比较了来自里约热内卢的114份HIV-1阳性血清对Bra-cons肽和其他V3肽（MN、SF2、HBX2、RF、MAL、ELI、Z6和一种科特迪瓦肽CI-cons）的血清学反应性。69份血清（60.5%）与属于B基因型的肽发生反应，其中10份血清也与属于A基因型（n = 7）和D基因型（n = 3）的肽发生反应。18份血清（15.8%）对Bra-cons肽有结合抗体。大量血清（n = 43；37.7%）对所测试的任何V3肽均无抗体。这一结果表明，具有异常V3环的HIV-1变体可能在里约热内卢传播。
