Pruckler J M, Pruckler J M, Ades E W
Biological Products Branch, Centers for Disease Control and Prevention, Atlanta, Ga 30333, USA.
Pathobiology. 1995;63(1):9-11. doi: 10.1159/000163929.
The identification of cell cultures contaminated with organisms from the class Mollicutes has led us to examine the effectiveness of polymerase chain reaction (PCR) for detecting these organisms in genomic DNA. We developed a previously identified nested PCR primer set and compared its ability to detect Mycoplasma with that of a commercially available PCR kit for detecting Mycoplasma. We found that although the commercial system detected and identified a few of the most common Mycoplasma species, the primer set (GPO-1, GPO-2, MGSO) detected the presence of all the common Mycoplasma species and many of the rare mycoplasma species previously encountered in tissue culture.
对被支原体类微生物污染的细胞培养物的鉴定，促使我们研究聚合酶链反应（PCR）在检测基因组DNA中这些微生物方面的有效性。我们开发了一套先前已鉴定的巢式PCR引物，并将其检测支原体的能力与一种市售的用于检测支原体的PCR试剂盒进行了比较。我们发现，尽管商业系统检测并鉴定出了一些最常见的支原体物种，但我们的引物组（GPO-1、GPO-2、MGSO）检测出了所有常见支原体物种以及许多先前在组织培养中遇到的罕见支原体物种的存在。
