Barcellini W, Rizzardi G P, Velati C, Borghi M O, Fain C, Lazzarin A, Meroni P L
Institute of Internal Medicine, Infectious Diseases and Immunopathology, University of Milan, Italy.
AIDS. 1995 Jul;9(7):691-4. doi: 10.1097/00002030-199507000-00004.
To study type 1 and type 2 cytokine patterns in HIV-negative high-risk intravenous drug users (IVDU).
We investigated interleukin (IL)-2, interferon (IFN)-gamma, IL-4, IL-6 and IL-10 production by phytohaemagglutinin (PHA)-stimulated and unstimulated peripheral blood mononuclear cell (PBMC) cultures from HIV-negative high-risk IVDU, HIV-negative controls and HIV-positive subjects.
Cytokine production was measured in supernatants using enzyme-linked immunosorbent assay (ELISA) in 10 HIV-negative high-risk IVDU, 25 HIV-negative controls, and 12 HIV-positive IVDU. We also determined spontaneous in vitro immunoglobulin (Ig) G and IgM production.
HIV-negative high-risk IVDU showed increased IFN-gamma and decreased IL-4, IL-10 and IL-2, although the latter was not significant compared with HIV-negative controls. Further, HIV-negative high-risk IVDU had reduced IgG production and impaired IgM-IgG switch.
The reduced IL-2 and IL-4 production suggest an impaired CD4+ T-cell function in HIV-negative high-risk IVDU. The increased IFN-gamma production along with the decreased type 2 cytokine profile is consistent with the hypothesis that protective immunity against HIV may reside in type 1 responses and cell-mediated immunity.
研究人类免疫缺陷病毒（HIV）阴性的高危静脉吸毒者（IVDU）中1型和2型细胞因子模式。
我们调查了HIV阴性的高危IVDU、HIV阴性对照者和HIV阳性受试者经植物血凝素（PHA）刺激及未刺激的外周血单个核细胞（PBMC）培养物中白细胞介素（IL）-2、干扰素（IFN）-γ、IL-4、IL-6和IL-10的产生情况。
采用酶联免疫吸附测定（ELISA）法检测10名HIV阴性的高危IVDU、25名HIV阴性对照者和12名HIV阳性IVDU的上清液中的细胞因子产生情况。我们还测定了体外自发免疫球蛋白（Ig）G和IgM的产生情况。
HIV阴性的高危IVDU显示IFN-γ增加，IL-4、IL-10和IL-2减少，尽管与HIV阴性对照者相比，后者差异不显著。此外，HIV阴性的高危IVDU的IgG产生减少，IgM-IgG转换受损。
IL-2和IL-4产生减少表明HIV阴性的高危IVDU的CD4+T细胞功能受损。IFN-γ产生增加以及2型细胞因子谱降低与以下假设一致，即针对HIV的保护性免疫可能存在于1型反应和细胞介导的免疫中。
